Horse CXC-Chemokine Receptor 3 ELISA Kit | CXCR3 elisa kit

Horse CXC-Chemokine Receptor 3 ELISA Kit

Cat. Num. AAA20248

• Gene Names: CXCR3; GPR9; MigR; CD182; CD183; Mig-R; CKR-L2; CMKAR3; IP10-R
• Reactivity: Horse
• Synonyms: CXC-Chemokine Receptor 3; Horse CXC-Chemokine Receptor 3 ELISA Kit; CXCR3 elisa kit

• Reactivity: Horse
• Specificity: No significant cross-reactivity or interference between Horse CXCR3 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 0.25ng/ml-8ng/ml.
• Sensitivity: 0.1ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated CXCR3 concentrations in Horse serum, plasma and other body fluids. Using Purified Horse CXCR3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CXCR3 and CXCR3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CXCR3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for CXCR3 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated CXCR3 concentrations in Horse serum, plasma and other body fluids. Using Purified Horse CXCR3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CXCR3 and CXCR3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CXCR3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 218563730
• NCBI GeneID: 2833
• NCBI Accession #: NP_001136269.1
• NCBI GenBank Nucleotide #: NM_001142797.1
• UniProt Accession #: P49682; O15185; Q7Z710; Q9P2T4; Q9P2T5; B2R982
• Molecular Weight: 40,660 Da
• NCBI Official Full Name: C-X-C chemokine receptor type 3 isoform 2
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) receptor 3
• NCBI Official Symbol: CXCR3
• NCBI Official Synonym Symbols: GPR9; MigR; CD182; CD183; Mig-R; CKR-L2; CMKAR3; IP10-R
• NCBI Protein Information: C-X-C chemokine receptor type 3; CXC-R3; CXCR-3; Mig receptor; IP10 receptor; IP-10 receptor; G protein-coupled receptor 9; chemokine (C-X-C) receptor 3; interferon-inducible protein 10 receptor
• UniProt Protein Name: C-X-C chemokine receptor type 3
• Protein Family: C-X-C chemokine receptor
• UniProt Gene Name: CXCR3
• UniProt Synonym Gene Names: GPR9; CXC-R3; CXCR-3
• UniProt Entry Name: CXCR3_HUMAN

NCBI Description

This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed CXCL9/Mig (monokine induced by interferon-g), CXCL10/IP10 (interferon-g-inducible 10 kDa protein) and CXCL11/I-TAC (interferon-inducible T cell a-chemoattractant). Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms (CXCR3-B) shows high affinity binding to chemokine, CXCL4/PF4 (PMID:12782716). [provided by RefSeq, Jun 2011]

Uniprot Description

CXCR3: Receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC). Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: Xq13

Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane

Molecular Function: C-X-C chemokine binding; chemokine binding; chemokine receptor activity; receptor activity; C-X-C chemokine receptor activity

Biological Process: positive regulation of chemotaxis; apoptosis; calcium-mediated signaling; positive regulation of cAMP metabolic process; chemotaxis; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; elevation of cytosolic calcium ion concentration; positive regulation of angiogenesis; cell surface receptor linked signal transduction; negative regulation of endothelial cell proliferation; positive regulation of cell proliferation; regulation of leukocyte migration; positive regulation of transcription from RNA polymerase II promoter; positive regulation of release of sequestered calcium ion into cytosol; angiogenesis; cell motility; cell adhesion; inflammatory response