Rat Filamin Binding LIM Protein 1 (FBLIM1) ELISA Kit | FBLIM1 elisa kit

Rat Filamin Binding LIM Protein 1 (FBLIM1) ELISA Kit

Cat. Num. AAA2024673

• Gene Names: FBLIM1; CAL; FBLP1; FBLP-1
• Reactivity: Rat
• Synonyms: Filamin Binding LIM Protein 1 (FBLIM1); Rat Filamin Binding LIM Protein 1 (FBLIM1) ELISA Kit; CAL; FBLP-1; FBLP1; Migfilin; Mitogen-inducible 2-interacting protein; FBLIM1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Filamin Binding LIM Protein 1 (FBLIM1).; No significant cross-reactivity or interference between Filamin Binding LIM Protein 1 (FBLIM1) and analogues was observed.
• Sequence Length: 373

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.058ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Filamin Binding LIM Protein 1 (FBLIM1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Filamin Binding LIM Protein 1 (FBLIM1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FBLIM1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Filamin Binding LIM Protein 1 (FBLIM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Filamin Binding LIM Protein 1 (FBLIM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Filamin Binding LIM Protein 1 (FBLIM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Filamin Binding LIM Protein 1 (FBLIM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for FBLIM1 elisa kit

Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 66932982
• NCBI GeneID: 54751
• NCBI Accession #: NP_060026.2
• NCBI GenBank Nucleotide #: NM_017556.2
• UniProt Accession #: Q8WUP2; Q5VVE0; Q5VVE1; Q8IX23; Q96T00; Q9UFD6; B3KNY0
• Molecular Weight: 30,770 Da
• NCBI Official Full Name: filamin-binding LIM protein 1 isoform a
• NCBI Official Synonym Full Names: filamin binding LIM protein 1
• NCBI Official Symbol: FBLIM1
• NCBI Official Synonym Symbols: CAL; FBLP1; FBLP-1
• NCBI Protein Information: filamin-binding LIM protein 1
• UniProt Protein Name: Filamin-binding LIM protein 1
• Protein Family: Filamin-binding LIM protein
• UniProt Gene Name: FBLIM1
• UniProt Synonym Gene Names: FBLP1; FBLP-1; MIG2-interacting protein
• UniProt Entry Name: FBLI1_HUMAN

NCBI Description

This gene encodes a protein with an N-terminal filamin-binding domain, a central proline-rich domain, and, multiple C-terminal LIM domains. This protein localizes at cell junctions and may link cell adhesion structures to the actin cytoskeleton. This protein may be involved in the assembly and stabilization of actin-filaments and likely plays a role in modulating cell adhesion, cell morphology and cell motility. This protein also localizes to the nucleus and may affect cardiomyocyte differentiation after binding with the CSX/NKX2-5 transcription factor. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

FBLIM1: Serves as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. Is implicated in cell shape modulation (spreading) and motility. May participate in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. May also regulate the assembly of filamin- containing signaling complexes that control actin assembly. Promotes dissociation of FLNA from ITGB3 and ITGB7. Promotes activation of integrins and regulates integrin-mediated cell-cell adhesion. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1p36.21

Cellular Component: focal adhesion; stress fiber; cell cortex; cytosol

Molecular Function: zinc ion binding; filamin binding

Biological Process: regulation of cell shape; cell-cell adhesion; regulation of integrin activation