Human Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP) ELISA Kit | MCSP elisa kit

Human Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP) ELISA Kit

Cat. Num. AAA2023986

• Gene Names: CSPG4; NG2; MCSP; MCSPG; MSK16; HMW-MAA; MEL-CSPG
• Reactivity: Human
• Synonyms: Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP); Human Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP) ELISA Kit; CSPG4; MSK16; HMW-MAA; MCSP; MCSPG; MEL-CSPG; NG2; Chondroitin Sulfate Proteoglycan 4; Melanoma chondroitin sulfate proteoglycan; MCSP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP).; No significant cross-reactivity or interference between Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP) and analogues was observed.
• Sequence Length: 2,322

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.056ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MCSP elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MCSP elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 1617314
• NCBI GeneID: 1464
• UniProt Accession #: Q6UVK1; Q92675; D3DW77
• Molecular Weight: 250,537 Da
• NCBI Official Full Name: melanoma-associated chondroitin sulfate proteoglycan (MCSP)
• NCBI Official Synonym Full Names: chondroitin sulfate proteoglycan 4
• NCBI Official Symbol: CSPG4
• NCBI Official Synonym Symbols: NG2; MCSP; MCSPG; MSK16; HMW-MAA; MEL-CSPG
• NCBI Protein Information: chondroitin sulfate proteoglycan 4
• UniProt Protein Name: Chondroitin sulfate proteoglycan 4
• UniProt Gene Name: CSPG4
• UniProt Synonym Gene Names: MCSP
• UniProt Entry Name: CSPG4_HUMAN

NCBI Description

A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq, Jul 2008]

Uniprot Description

NG2: a transmembrane chondrotin sulfate proteoglycan expressed on several types of immature progenitor cells and human malignant melanoma cells. Plays a role in cell proliferation and motility of endothelial cells during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. May regulate MPP16-dependent degradation and invasion of type I collagen participating to melanoma cells invasion properties. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through Cdc42, ACK1 and P130Cas. May activate FAK and ERK signaling cascades. PKC-alpha-mediated NG2 phosphorylation may be a key step for initiating cell polarization and motility.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q24.2

Cellular Component: lysosomal lumen; focal adhesion; cell surface; Golgi lumen; integral to plasma membrane; apical plasma membrane; extracellular region

Molecular Function: signal transducer activity; protein kinase binding

Biological Process: chondroitin sulfate metabolic process; cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; activation of MAPK activity; carbohydrate metabolic process; chondroitin sulfate catabolic process; tissue remodeling; pathogenesis; angiogenesis; glial cell migration; transmembrane receptor protein tyrosine kinase signaling pathway; dermatan sulfate biosynthetic process