Wingless Type MMTV Integration Site Family, Member 4 (WNT4) ELISA Kit | WNT4 elisa kit

Rat Wingless Type MMTV Integration Site Family, Member 4 (WNT4) ELISA Kit

Cat. Num. AAA2023235

• Gene Names: WNT4; WNT-4; SERKAL
• Reactivity: Rat
• Synonyms: Wingless Type MMTV Integration Site Family; Member 4 (WNT4); Rat Wingless Type MMTV Integration Site Family; Member 4 (WNT4) ELISA Kit; WNT4 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 4 (WNT4).; No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 4 (WNT4) and analogues was observed.
• Sequence Length: 296

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 4 (WNT4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 4 (WNT4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for WNT4 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 4 (WNT4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 4 (WNT4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wingless Type MMTV Integration Site Family, Member 4 (WNT4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Wingless Type MMTV Integration Site Family, Member 4 (WNT4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for WNT4 elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 119615416
• NCBI GeneID: 54361
• UniProt Accession #: P56705; Q5TZQ0; Q96T81; Q9BXF5; Q9H1J8; Q9UJM2; B4DJF9
• Molecular Weight: 32,954 Da
• NCBI Official Full Name: wingless-type MMTV integration site family, member 4
• NCBI Official Synonym Full Names: wingless-type MMTV integration site family, member 4
• NCBI Official Symbol: WNT4
• NCBI Official Synonym Symbols: WNT-4; SERKAL
• NCBI Protein Information: protein Wnt-4
• UniProt Protein Name: Protein Wnt-4
• UniProt Gene Name: WNT4
• UniProt Entry Name: WNT4_HUMAN

NCBI Description

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and a nuclear receptor known to antagonize the testis-determining factor play a concerted role in both the control of female development and the prevention of testes formation. This gene and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue. Mutations in this gene can result in Rokitansky-Kuster-Hauser syndrome and in SERKAL syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

WNT4: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Overexpression may be associated with abnormal proliferation in human breast tissue. Defects in WNT4 are a cause of Rokitansky-Kuster-Hauser syndrome (RKH syndrome); also called Mayer- Rokitansky-Kuster-Hauser syndrome (MRKH syndrome or MRKH anomaly). RKH syndrome is characterized by utero-vaginal atresia in otherwise phenotypically normal female with a normal 46,XX karyotype. Anomalies of the genital tract range from upper vaginal atresia to total Muellerian agenesis with urinary tract abnormalities. It has an incidence of approximately 1 in 5'000 newborn girls. Defects in WNT4 are the cause of female sex reversal with dysgenesis of kidneys, adrenals, and lungs (SERKAL); also known as SERKAL syndrome. Defects in WNT4 are the cause of Muellerian aplasia (MULLAPL). Belongs to the Wnt family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36.23-p35.1

Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; endoplasmic reticulum lumen; Golgi lumen; cytoplasm; plasma membrane; extracellular region

Molecular Function: frizzled binding; transcription corepressor activity; receptor agonist activity

Biological Process: negative regulation of fibroblast growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; positive regulation of collagen biosynthetic process; thyroid stimulating hormone secreting cell differentiation; androgen biosynthetic process; Wnt receptor signaling pathway through beta-catenin; somatotropin secreting cell differentiation; neuron differentiation; regulation of cell-cell adhesion; positive regulation of focal adhesion formation; positive regulation of aldosterone biosynthetic process; positive regulation of stress fiber formation; embryonic epithelial tube formation; kidney development; negative regulation of cell migration; female gonad development; cell fate commitment; positive regulation of meiosis; adrenal gland development; male gonad development; immature T cell proliferation in the thymus; liver development; positive regulation of bone mineralization; cellular response to starvation; positive regulation of osteoblast differentiation; negative regulation of cell differentiation; protein palmitoylation; ureteric bud branching; epithelial to mesenchymal transition; smooth muscle cell differentiation; female sex determination; negative regulation of transcription, DNA-dependent; oocyte development

Disease: Mullerian Aplasia And Hyperandrogenism; 46,xx Sex Reversal With Dysgenesis Of Kidneys, Adrenals, And Lungs