Mouse ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3) ELISA Kit | ATP1b3 elisa kit

Mouse ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3) ELISA Kit

Cat. Num. AAA2022641

• Gene Names: ATP1B3; CD298; ATPB-3
• Reactivity: Mouse
• Synonyms: ATPase; Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3); Mouse ATPase; Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3) ELISA Kit; CD298; ATP1-B3; ATPB-3; Sodium/potassium-transporting ATPase subunit beta-3; ATP1b3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3).; No significant cross-reactivity or interference between ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3) and analogues was observed.
• Sequence Length: 193

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.066ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATP1b3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ATP1b3 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 119599395
• NCBI GeneID: 483
• UniProt Accession #: P54709; B7Z1N7
• Molecular Weight: 21,713 Da
• NCBI Official Full Name: ATPase, Na+/K+ transporting, beta 3 polypeptide, isoform CRA_f
• NCBI Official Synonym Full Names: ATPase, Na+/K+ transporting, beta 3 polypeptide
• NCBI Official Symbol: ATP1B3
• NCBI Official Synonym Symbols: CD298; ATPB-3
• NCBI Protein Information: sodium/potassium-transporting ATPase subunit beta-3
• UniProt Protein Name: Sodium/potassium-transporting ATPase subunit beta-3
• UniProt Gene Name: ATP1B3
• UniProt Synonym Gene Names: ATPB-3
• UniProt Entry Name: AT1B3_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 3 subunit. This gene encodes a beta 3 subunit. A pseudogene exists for this gene, and it is located on chromosome 2. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP1B3: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-3 subunit is not known. Belongs to the X(+)/potassium ATPases subunit beta family.

Protein type: Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q23

Cellular Component: plasma membrane; melanosome; caveola; sodium:potassium-exchanging ATPase complex

Molecular Function: ATPase activator activity; sodium:potassium-exchanging ATPase activity; ATPase binding

Biological Process: cellular sodium ion homeostasis; potassium ion import; protein stabilization; positive regulation of ATPase activity; transport; blood coagulation; transmembrane transport; cellular potassium ion homeostasis; leukocyte migration