Porcine Insulin Like Growth Factor Binding Protein 2 (IGFBP2) ELISA Kit | IGFBP2 elisa kit

Porcine Insulin Like Growth Factor Binding Protein 2 (IGFBP2) ELISA Kit

Cat. Num. AAA2022564

• Gene Names: IGFBP2; IBP2; IGF-BP53
• Reactivity: Porcine
• Synonyms: Insulin Like Growth Factor Binding Protein 2 (IGFBP2); Porcine Insulin Like Growth Factor Binding Protein 2 (IGFBP2) ELISA Kit; IBP2; IGF-BP53; IGFBP2 elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor Binding Protein 2 (IGFBP2).; No significant cross-reactivity or interference between Insulin Like Growth Factor Binding Protein 2 (IGFBP2) and analogues was observed.
• Sequence Length: 325

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 0.469-30ng/mL
• Sensitivity: < 0.18ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor Binding Protein 2 (IGFBP2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor Binding Protein 2 (IGFBP2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IGFBP2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor Binding Protein 2 (IGFBP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor Binding Protein 2 (IGFBP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor Binding Protein 2 (IGFBP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Like Growth Factor Binding Protein 2 (IGFBP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for IGFBP2 elisa kit

Tumor immunity; Endocrinology

NCBI and Uniprot Product Information

• NCBI GI #: 55925576
• NCBI GeneID: 3485
• NCBI Accession #: NP_000588.2
• NCBI GenBank Nucleotide #: NM_000597.2
• UniProt Accession #: P18065; Q14619; Q9UCL3
• Molecular Weight: 34,814 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein 2
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 2, 36kDa
• NCBI Official Symbol: IGFBP2
• NCBI Official Synonym Symbols: IBP2; IGF-BP53
• NCBI Protein Information: insulin-like growth factor-binding protein 2
• UniProt Protein Name: Insulin-like growth factor-binding protein 2
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: IGFBP2
• UniProt Synonym Gene Names: BP2; IBP2; IBP-2; IGF-binding protein 2; IGFBP-2
• UniProt Entry Name: IBP2_HUMAN

Uniprot Description

IGFBP2: Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Binds IGF2 more than IGF1.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: extracellular space; apical plasma membrane; extracellular region; cytoplasmic vesicle

Molecular Function: protein binding; insulin-like growth factor I binding; insulin-like growth factor II binding

Biological Process: response to drug; response to retinoic acid; regulation of insulin-like growth factor receptor signaling pathway; response to lithium ion; response to glucocorticoid stimulus; female pregnancy; signal transduction; positive regulation of activated T cell proliferation; response to estradiol stimulus; cellular response to hormone stimulus; cellular protein metabolic process; response to mechanical stimulus; regulation of cell growth; response to nutrient; aging