Rat Bone Morphogenetic Protein 6 (BMP6) ELISA Kit | BMP6 elisa kit

Rat Bone Morphogenetic Protein 6 (BMP6) ELISA Kit

Cat. Num. AAA2022029

• Gene Names: BMP6; VGR; VGR1
• Reactivity: Rat
• Synonyms: Bone Morphogenetic Protein 6 (BMP6); Rat Bone Morphogenetic Protein 6 (BMP6) ELISA Kit; VGR; VGR1; Vegetal Related Growth Factor(TGFB-Related); VG-1-related protein; BMP6 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 6 (BMP6).; No significant cross-reactivity or interference between Bone Morphogenetic Protein 6 (BMP6) and analogues was observed.
• Sequence Length: 513

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 15.6-1000pg/mL
• Sensitivity: < 6.2pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 6 (BMP6) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 6 (BMP6) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BMP6 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 6 (BMP6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 6 (BMP6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein 6 (BMP6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bone Morphogenetic Protein 6 (BMP6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for BMP6 elisa kit

Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 3492993
• NCBI GeneID: 654
• UniProt Accession #: P22004; Q5TCP3
• Molecular Weight: 57,226 Da
• NCBI Official Full Name: bone morphogenetic protein 6, partial
• NCBI Official Synonym Full Names: bone morphogenetic protein 6
• NCBI Official Symbol: BMP6
• NCBI Official Synonym Symbols: VGR; VGR1
• NCBI Protein Information: bone morphogenetic protein 6
• UniProt Protein Name: Bone morphogenetic protein 6
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP6
• UniProt Synonym Gene Names: VGR; BMP-6; VG-1-R; VGR-1
• UniProt Entry Name: BMP6_HUMAN

NCBI Description

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development. In addition, the fact that this BMP is closely related to BMP5 and BMP7 has lead to speculation of possible bone inductive activity. [provided by RefSeq, Jul 2008]

Uniprot Description

BMP6: Induces cartilage and bone formation. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p24-p23

Cellular Component: extracellular space; cytoplasm

Molecular Function: growth factor activity; protein heterodimerization activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: cellular iron ion homeostasis; response to glucocorticoid stimulus; regulation of apoptosis; BMP signaling pathway; response to magnesium ion; positive regulation of endothelial cell differentiation; positive regulation of aldosterone biosynthetic process; male genitalia development; kidney development; inflammatory response; skeletal development; response to iron ion; endochondral ossification; response to retinoic acid; positive regulation of bone mineralization; osteoblast differentiation; positive regulation of osteoblast differentiation; positive regulation of chondrocyte differentiation; eye development; positive regulation of protein secretion; regulation of MAPKKK cascade; cartilage development; immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; response to activity; positive regulation of neuron differentiation; positive regulation of epithelial cell proliferation; growth