Human Coenzyme Q10 Homolog B (COQ10B) ELISA Kit | COQ10B elisa kit

Human Coenzyme Q10 Homolog B (COQ10B) ELISA Kit

Cat. Num. AAA2021992

• Reactivity: Human
• Synonyms: Coenzyme Q10 Homolog B (COQ10B); Human Coenzyme Q10 Homolog B (COQ10B) ELISA Kit; Coenzyme Q-binding protein COQ10 homolog B; mitochondrial; COQ10B elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Coenzyme Q10 Homolog B (COQ10B).; No significant cross-reactivity or interference between Coenzyme Q10 Homolog B (COQ10B) and analogues was observed.
• Sequence Length: 215

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.127ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coenzyme Q10 Homolog B (COQ10B) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coenzyme Q10 Homolog B (COQ10B) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COQ10B elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coenzyme Q10 Homolog B (COQ10B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coenzyme Q10 Homolog B (COQ10B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coenzyme Q10 Homolog B (COQ10B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coenzyme Q10 Homolog B (COQ10B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for COQ10B elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 151101386
• NCBI GeneID: 93058
• NCBI Accession #: NP_001092807.1
• NCBI GenBank Nucleotide #: NM_001099337.1
• UniProt Accession #: Q96MF6; Q6GMR6; Q6UWB9; Q86X16; Q8TAL2; Q96MF1; Q9BUP4
• Molecular Weight: 24,460 Da
• NCBI Official Full Name: coenzyme Q-binding protein COQ10 homolog A, mitochondrial isoform b
• NCBI Official Synonym Full Names: coenzyme Q10A
• NCBI Official Symbol: COQ10A
• NCBI Protein Information: coenzyme Q-binding protein COQ10 homolog A, mitochondrial
• UniProt Protein Name: Coenzyme Q-binding protein COQ10 homolog A, mitochondrial
• Protein Family: Coenzyme Q-binding protein
• UniProt Gene Name: COQ10A
• UniProt Entry Name: CQ10A_HUMAN

Uniprot Description

COQ10A: Required for the function of coenzyme Q in the respiratory chain. May serve as a chaperone or may be involved in the transport of Q6 from its site of synthesis to the catalytic sites of the respiratory complexes (Probable). Belongs to the COQ10 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: mitochondrial inner membrane