Mouse Topoisomerase II (TOP2) ELISA Kit | TOP2 elisa kit

Mouse Topoisomerase II (TOP2) ELISA Kit

Cat. Num. AAA2020277

• Reactivity: Mouse
• Synonyms: Topoisomerase II (TOP2); Mouse Topoisomerase II (TOP2) ELISA Kit; DNA topoisomerase 2-alpha; DNA topoisomerase II; alpha isozyme; TOP2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Topoisomerase II (TOP2).; No significant cross-reactivity or interference between Topoisomerase II (TOP2) and analogues was observed.
• Sequence Length: 886

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.116ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Topoisomerase II (TOP2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Topoisomerase II (TOP2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TOP2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Topoisomerase II (TOP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Topoisomerase II (TOP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Topoisomerase II (TOP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Topoisomerase II (TOP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for TOP2 elisa kit

Enzyme & Kinase; Tumor immunity; Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 339810
• Molecular Weight: 182,663 Da
• NCBI Official Full Name: topoisomerase II, partial
• UniProt Protein Name: DNA topoisomerase 2-beta
• Protein Family: DNA topoisomerase
• UniProt Gene Name: TOP2B
• UniProt Entry Name: TOP2B_HUMAN

Uniprot Description

TOP2B: Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Indirectly involved in vitamin D- coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR- mediated transrepression of the CYP27B1 gene. Belongs to the type II topoisomerase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; EC 5.99.1.3; DNA-binding; Cell development/differentiation; Topoisomerase; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p24

Cellular Component: nucleoplasm; heterochromatin; DNA topoisomerase complex (ATP-hydrolyzing); cytoplasm; nucleolus; cytosol; nucleus

Molecular Function: protein C-terminus binding; DNA topoisomerase (ATP-hydrolyzing) activity; enzyme binding; protein kinase C binding; protein heterodimerization activity; metal ion binding; histone deacetylase binding; chromatin binding; DNA bending activity; ATP binding

Biological Process: DNA unwinding during replication; mitotic recombination; DNA topological change; axonogenesis; forebrain development; neuron migration; sister chromatid segregation; resolution of meiotic joint molecules as recombinants