Human Ficolin 1 (FCN1) ELISA Kit | FCN1 elisa kit

Human Ficolin 1 (FCN1) ELISA Kit

Cat. Num. AAA2020259

• Gene Names: FCN1; FCNM
• Reactivity: Human
• Synonyms: Ficolin 1 (FCN1); Human Ficolin 1 (FCN1) ELISA Kit; FCNM; Collagen/fibrinogen domain-containing protein 1; Ficolin-alpha; Ficolin-A; M-ficolin; FCN1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ficolin 1 (FCN1).; No significant cross-reactivity or interference between Ficolin 1 (FCN1) and analogues was observed.
• Sequence Length: 326

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.066ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ficolin 1 (FCN1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ficolin 1 (FCN1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FCN1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ficolin 1 (FCN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ficolin 1 (FCN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ficolin 1 (FCN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ficolin 1 (FCN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for FCN1 elisa kit

Metabolic pathway; Infection immunity; Bone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 8051584
• NCBI GeneID: 2219
• NCBI Accession #: NP_001994.2
• NCBI GenBank Nucleotide #: NM_002003.3
• UniProt Accession #: O00602; Q5VYV5; Q92596
• Molecular Weight: 35,078 Da
• NCBI Official Full Name: ficolin-1
• NCBI Official Synonym Full Names: ficolin (collagen/fibrinogen domain containing) 1
• NCBI Official Symbol: FCN1
• NCBI Official Synonym Symbols: FCNM
• NCBI Protein Information: ficolin-1
• UniProt Protein Name: Ficolin-1
• Protein Family: Ficolin
• UniProt Gene Name: FCN1
• UniProt Synonym Gene Names: FCNM
• UniProt Entry Name: FCN1_HUMAN

NCBI Description

The ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. The collagen-like and the fibrinogen-like domains are also found separately in other proteins such as complement protein C1q, C-type lectins known as collectins, and tenascins. However, all these proteins recognize different targets, and are functionally distinct. Ficolin 1 encoded by FCN1 is predominantly expressed in the peripheral blood leukocytes, and has been postulated to function as a plasma protein with elastin-binding activity. [provided by RefSeq, Jul 2008]

Uniprot Description

FCN1: Complement-activating lectin and pattern recognition receptor. Binds GlcNAc. Binds preferentially to 9-O-acetylated 2- 6-linked sialic acid derivatives and to various glycans containing sialic acid engaged in a 2-3 linkage. Belongs to the ficolin lectin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: extrinsic to external side of plasma membrane; collagen; extracellular region

Molecular Function: protein binding; G-protein-coupled receptor binding; metal ion binding; carbohydrate binding; sialic acid binding; pattern recognition receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of virion penetration into host cell; cell surface pattern recognition receptor signaling pathway; innate immune response; complement activation, lectin pathway; recognition of apoptotic cell; complement activation