Human FMS Like Tyrosine Kinase 3 (Flt3) ELISA Kit | Flt3 elisa kit

Human FMS Like Tyrosine Kinase 3 (Flt3) ELISA Kit

Cat. Num. AAA2019825

• Gene Names: Flt3l; Ly72L; Flt3lg
• Reactivity: Human
• Synonyms: FMS Like Tyrosine Kinase 3 (Flt3); Human FMS Like Tyrosine Kinase 3 (Flt3) ELISA Kit; CD135; STK1; FLK2; Fms-Related Tyrosine Kinase 3; Stem Cell Tyrosine Kinase 1; FL Cytokine Receptor; Fetal Liver Kinase-2; Flt3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of FMS Like Tyrosine Kinase 3 (Flt3).; No significant cross-reactivity or interference between FMS Like Tyrosine Kinase 3 (Flt3) and analogues was observed.
• Sequence Length: 232

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.125ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FMS Like Tyrosine Kinase 3 (Flt3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FMS Like Tyrosine Kinase 3 (Flt3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Flt3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FMS Like Tyrosine Kinase 3 (Flt3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to FMS Like Tyrosine Kinase 3 (Flt3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FMS Like Tyrosine Kinase 3 (Flt3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FMS Like Tyrosine Kinase 3 (Flt3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for Flt3 elisa kit

CD & Adhesion molecule; Hematology

NCBI and Uniprot Product Information

• NCBI GI #: 227430331
• NCBI GeneID: 14256
• NCBI Accession #: NP_038548.3
• NCBI GenBank Nucleotide #: NM_013520.3
• Molecular Weight: 28,259 Da
• NCBI Official Full Name: fms-related tyrosine kinase 3 ligand
• NCBI Official Synonym Full Names: FMS-like tyrosine kinase 3 ligand
• NCBI Official Symbol: Flt3l
• NCBI Official Synonym Symbols: Ly72L; Flt3lg
• NCBI Protein Information: fms-related tyrosine kinase 3 ligand
• UniProt Protein Name: Fms-related tyrosine kinase 3 ligand
• Protein Family: Receptor-type tyrosine-protein kinase
• UniProt Gene Name: Flt3lg
• UniProt Synonym Gene Names: Flt3l; Flt3 ligand; Flt3L
• UniProt Entry Name: FLT3L_MOUSE

Uniprot Description

FLT3LG: Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligand, receptor tyrosine kinase; Cytokine; Membrane protein, integral

Cellular Component: extracellular space; cell surface; membrane; integral to membrane; plasma membrane; extracellular region

Molecular Function: protein homodimerization activity; cytokine activity; receptor tyrosine kinase binding

Biological Process: positive regulation of natural killer cell differentiation; regulation of myeloid dendritic cell activation; lymphocyte differentiation; embryonic hemopoiesis; positive regulation of cell proliferation; positive regulation of protein amino acid phosphorylation; positive regulation of myoblast differentiation