Human Protease Activated Receptor 4 (PAR4) ELISA Kit | PAR4 elisa kit

Human Protease Activated Receptor 4 (PAR4) ELISA Kit

Cat. Num. AAA2019447

• Gene Names: F2RL3; PAR4
• Reactivity: Human
• Synonyms: Protease Activated Receptor 4 (PAR4); Human Protease Activated Receptor 4 (PAR4) ELISA Kit; F2RL3; PAR-4; Coagulation Factor II Thrombin Receptor-Like 3; Coagulation factor II receptor-like 3; PAR4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Protease Activated Receptor 4 (PAR4).; No significant cross-reactivity or interference between Protease Activated Receptor 4 (PAR4) and analogues was observed.
• Sequence Length: 385

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.122ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protease Activated Receptor 4 (PAR4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protease Activated Receptor 4 (PAR4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PAR4 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protease Activated Receptor 4 (PAR4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protease Activated Receptor 4 (PAR4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protease Activated Receptor 4 (PAR4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protease Activated Receptor 4 (PAR4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PAR4 elisa kit

Hematology

NCBI and Uniprot Product Information

• NCBI GI #: 89353783
• NCBI GeneID: 9002
• NCBI Accession #: NP_003941.2
• NCBI GenBank Nucleotide #: NM_003950.2
• UniProt Accession #: Q96RI0; O76067; Q6DK42
• Molecular Weight: 41,133 Da
• NCBI Official Full Name: proteinase-activated receptor 4
• NCBI Official Synonym Full Names: coagulation factor II (thrombin) receptor-like 3
• NCBI Official Symbol: F2RL3
• NCBI Official Synonym Symbols: PAR4
• NCBI Protein Information: proteinase-activated receptor 4
• UniProt Protein Name: Proteinase-activated receptor 4
• UniProt Gene Name: F2RL3
• UniProt Synonym Gene Names: PAR4; PAR-4
• UniProt Entry Name: PAR4_HUMAN

NCBI Description

Coagulation factor II (thrombin) receptor-like 3 (F2RL3) is a member of the large family of 7-transmembrane-region receptors that couple to guanosine-nucleotide-binding proteins. F2RL3 is also a member of the protease-activated receptor family. F2RL3 is activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor. F2RL3 is activated by thrombin and trypsin. [provided by RefSeq, Jul 2008]

Uniprot Description

PAR4: a G-protein coupled receptor for activated thrombin or trypsin. Coupled to G proteins that stimulate phosphoinositide hydrolysis. Plays a role in platelets activation. Thrombin requires PAR4 for full spreading on a fibrinogen matrix. Widely expressed, with highest levels in lung, pancreas, thyroid, testis and small intestine. Not expressed in brain, kidney, spinal cord and peripheral blood leukocytes. Also detected in platelets. A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.

Protein type: Receptor, GPCR; Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 19p12

Cellular Component: integral to plasma membrane; extracellular region; plasma membrane

Molecular Function: thrombin receptor activity

Biological Process: platelet activation; platelet dense granule organization and biogenesis; response to wounding; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of release of sequestered calcium ion into cytosol; blood coagulation; signal transduction