Rat Period Circadian Protein 2 (PER2) ELISA Kit | PER2 elisa kit

Rat Period Circadian Protein 2 (PER2) ELISA Kit

Cat. Num. AAA2019443

• Gene Names: PER2; FASPS; FASPS1
• Reactivity: Rat
• Synonyms: Period Circadian Protein 2 (PER2); Rat Period Circadian Protein 2 (PER2) ELISA Kit; FASPS; Circadian clock protein PERIOD 2; PER2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Period Circadian Protein 2 (PER2).; No significant cross-reactivity or interference between Period Circadian Protein 2 (PER2) and analogues was observed.
• Sequence Length: 1255

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.101ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Period Circadian Protein 2 (PER2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Period Circadian Protein 2 (PER2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PER2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Period Circadian Protein 2 (PER2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Period Circadian Protein 2 (PER2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Period Circadian Protein 2 (PER2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Period Circadian Protein 2 (PER2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PER2 elisa kit

Neuroscience; Circadian rhythm

NCBI and Uniprot Product Information

• NCBI GI #: 12707562
• NCBI GeneID: 8864
• NCBI Accession #: NP_073728.1
• NCBI GenBank Nucleotide #: NM_022817.2
• UniProt Accession #: O15055; Q4ZG49; Q6DT41; Q9UQ45; A2I2P7
• Molecular Weight: 45,175 Da
• NCBI Official Full Name: period circadian protein homolog 2
• NCBI Official Synonym Full Names: period circadian clock 2
• NCBI Official Symbol: PER2
• NCBI Official Synonym Symbols: FASPS; FASPS1
• NCBI Protein Information: period circadian protein homolog 2
• UniProt Protein Name: Period circadian protein homolog 2
• Protein Family: Period circadian protein
• UniProt Gene Name: PER2
• UniProt Synonym Gene Names: KIAA0347; hPER2
• UniProt Entry Name: PER2_HUMAN

NCBI Description

This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene may increase the risk of getting certain cancers and have been linked to sleep disorders. [provided by RefSeq, Jan 2014]

Uniprot Description

PER2: a transcription factor belonging to the basic helix-loop-helix (bHLH) family. A member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. Circadian expression in the suprachiasmatic nucleus continues in constant darkness, and a shift in the light/dark cycle evokes a proportional shift of gene expression in the suprachiasmatic nucleus. Behaves as a negative element in circadian transcriptional loop. Does not appear to bind DNA, suggesting indirect transcriptional inhibition. Expression oscillates with a 24 hr rhythm in the suprachiasmatic nucleus and the whole eyes. Oscillations are maintained under constant darkness and are responsive to changes of the light/dark cycles. There is a 4 hour time delay between per1 and per2 oscillations. The expression rhythms appear to originate from retina.

Protein type: Transcription factor; Nuclear receptor co-regulator; Nucleolus

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: nucleoplasm; perinuclear region of cytoplasm; cytoplasm; nucleolus; nucleus

Molecular Function: signal transducer activity; protein binding; ubiquitin binding; histone deacetylase binding; transcription coactivator activity; kinase binding; nuclear hormone receptor binding

Biological Process: circadian rhythm; glycogen biosynthetic process; transcription, DNA-dependent; regulation of cell cycle; negative regulation of transcription from RNA polymerase II promoter; regulation of circadian rhythm; fatty acid metabolic process; signal transduction; regulation of glutamate uptake during transmission of nerve impulse; gluconeogenesis; regulation of neurogenesis; regulation of vasoconstriction; white fat cell differentiation; lactate biosynthetic process; negative regulation of circadian rhythm; negative regulation of protein ubiquitination; circadian regulation of gene expression; negative regulation of transcription, DNA-dependent; regulation of insulin secretion

Disease: Advanced Sleep Phase Syndrome, Familial, 1