Interleukin 27 Receptor Alpha (IL27Ra) Recombinant Protein | IL27Ra recombinant protein

Recombinant Interleukin 27 Receptor Alpha (IL27Ra)

Cat. Num. AAA2018600

• Gene Names: IL27RA; CRL1; TCCR; WSX1; IL27R; IL-27RA; zcytor1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Interleukin 27 Receptor Alpha (IL27Ra); Recombinant Interleukin 27 Receptor Alpha (IL27Ra); IL27Ra recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- ETQMKPNA PRLGPDVDFS EDDPLEATVH WAPPTWPSHK VLICQFHYRR CQEAAWTLLE PELKTIPLTP VEIQDLELAT GYKVYGRCRM EKEEDLWGEW SPILSFQTPP SAPKDVWVSG NLCGTPGGEE PLLLWKAPGP CVQVSYKVWF WVGGRELSPE GITCCCSLIP SGAEWARVSA VNATSWEPLTNLSLVCLDSA SAPRSVAVSS IAGSTELLVT WQP
• Sequence Length: 636

• Applicable Applications for IL27Ra recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Glu123~Pro343 (Accession # Q6UWB1) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Membrane; Single-pass type I membrane protein
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 5.4
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4759328
• NCBI GeneID: 9466
• NCBI Accession #: NP_004834.1
• NCBI GenBank Nucleotide #: NM_004843.3
• UniProt Accession #: Q6UWB1; O60624; A0N0L1
• Molecular Weight: 28.2kDa
• NCBI Official Full Name: interleukin-27 receptor subunit alpha
• NCBI Official Synonym Full Names: interleukin 27 receptor, alpha
• NCBI Official Symbol: IL27RA
• NCBI Official Synonym Symbols: CRL1; TCCR; WSX1; IL27R; IL-27RA; zcytor1
• NCBI Protein Information: interleukin-27 receptor subunit alpha; IL-27R-alpha; IL-27R subunit alpha; cytokine receptor WSX-1; cytokine receptor-like 1; class I cytokine receptor; IL-27 receptor subunit alpha; T-cell cytokine receptor type 1; type I T-cell cytokine receptor
• UniProt Protein Name: Interleukin-27 receptor subunit alpha
• Protein Family: Interleukin-27 receptor
• UniProt Gene Name: IL27RA
• UniProt Synonym Gene Names: CRL1; TCCR; WSX1; IL-27 receptor subunit alpha; IL-27R subunit alpha; IL-27R-alpha; IL-27RA; TCCR
• UniProt Entry Name: I27RA_HUMAN

NCBI Description

In mice, CD4+ helper T-cells differentiate into type 1 (Th1) cells, which are critical for cell-mediated immunity, predominantly under the influence of IL12. Also, IL4 influences their differentiation into type 2 (Th2) cells, which are critical for most antibody responses. Mice deficient in these cytokines, their receptors, or associated transcription factors have impaired, but are not absent of, Th1 or Th2 immune responses. This gene encodes a protein which is similar to the mouse T-cell cytokine receptor Tccr at the amino acid level, and is predicted to be a glycosylated transmembrane protein. [provided by RefSeq, Jul 2008]

Uniprot Description

IL27RA: Receptor for IL27. Requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. Involved in the regulation of Th1- type immune responses. Also appears to be involved in innate defense mechanisms. Belongs to the type I cytokine receptor family. Type 2 subfamily.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.11

Cellular Component: integral to plasma membrane

Molecular Function: interleukin-27 receptor activity; transmembrane receptor activity

Biological Process: positive regulation of interferon-gamma production; defense response to Gram-positive bacterium; cell surface receptor linked signal transduction; negative regulation of T-helper 2 type immune response; positive regulation of T-helper 1 type immune response; immune response; regulation of isotype switching to IgG isotypes