Complement Component 8b (C8b) Recombinant Protein | C8b recombinant protein

Recombinant Complement Component 8b (C8b)

Cat. Num. AAA2018596

• Gene Names: C8b; AI595927; 4930439B20Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Complement Component 8b (C8b); Recombinant Complement Component 8b (C8b); C8b recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- CQREMEQYWA IDRLASGINL FTNTFEGPVL DHRYYAGGCS PHYILDTNFR KPYNVESYTP QTKCEYEFTL TEYESYSDFE RLVIEKKTHM FNFTSGFKVD GVMDLGIKVE SNEGKNYVTR TKRFAHTQSK FLHARSVLEV AHYKLKSRSL MLHYEFLQRV KSLPLEYSYG EYRDLLRDFG THFITEAVLG GIYEYTLIMN KDAMEQGDYT LSHVTACAGG SFGIGGMVYK VYVKVGVSAK KCSDIMKEIN ERNKRSTMVE DLVVLVRGGT SEDITALAYK ELPTPELMEA WGDAVKYNPA IIKIKAEPLY ELVTATDFAYSSTVKQNLKK ALEEFQSEVS SCR
• Sequence Length: 589

• Applicable Applications for C8b recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Cys161~Arg503 (Accession # Q8BH35) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Secreted
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.8
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 33563297
• NCBI GeneID: 110382
• NCBI Accession #: NP_598643.1
• NCBI GenBank Nucleotide #: NM_133882.2
• UniProt Accession #: Q8BH35; Q4VAH1; Q8CHJ5; Q8CHJ6; Q8VC14
• Molecular Weight: 42.8kDa
• NCBI Official Full Name: complement component C8 beta chain
• NCBI Official Synonym Full Names: complement component 8, beta polypeptide
• NCBI Official Symbol: C8b
• NCBI Official Synonym Symbols: AI595927; 4930439B20Rik
• NCBI Protein Information: complement component C8 beta chain; complement component 8 subunit beta; complement component 8, beta subunit
• UniProt Protein Name: Complement component C8 beta chain
• Protein Family: Complement component
• UniProt Gene Name: C8b
• UniProt Entry Name: CO8B_MOUSE

Uniprot Description

C8B: Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. Defects in C8B are a cause of complement component 8 deficiency type 2 (C8D2). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. Belongs to the complement C6/C7/C8/C9 family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: membrane attack complex; extracellular space; extracellular region; vesicle

Molecular Function: protein complex binding

Biological Process: immune system process; cytolysis; complement activation, alternative pathway; innate immune response; immune response; complement activation, classical pathway