SDS-Page
Secretogranin II (SCG2) Recombinant Protein | SCG2 recombinant protein
Recombinant Secretogranin II (SCG2)
Gene Names
Scg2; Chgc; SgII
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Secretogranin II (SCG2); Recombinant Secretogranin II (SCG2); SCG2 recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGS-AVLLLIHLIF LISGAEAASF QRNQLLQKEP DLRLENVQKF PSPEMIRALE YIEKLRQQAH REESSPDYNP YQGVSVPLQL KENGEESHLA ESSRDALSED EWMRIILEAL RQAENEPPSA PKENKPYALNLEKNFPVDTP DDYETQQWPE RKLKHMRFPL MYEENSRENP FKRTNEI
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGS-AVLLLIHLIF LISGAEAASF QRNQLLQKEP DLRLENVQKF PSPEMIRALE YIEKLRQQAH REESSPDYNP YQGVSVPLQL KENGEESHLA ESSRDALSED EWMRIILEAL RQAENEPPSA PKENKPYALNLEKNFPVDTP DDYETQQWPE RKLKHMRFPL MYEENSRENP FKRTNEI
Sequence Length
617
Applicable Applications for SCG2 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Mus musculus (Mouse)
Expression System
Prokaryotic expression
Residues
Ala11~Ile187 (Accession # Q03517) with two N-terminal Tags, His-tag and GST-tag
Subcellular Location
Secreted
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Predicted Isoelectric Point
5.8
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52.9kDa
NCBI Official Full Name
secretogranin-2
NCBI Official Synonym Full Names
secretogranin II
NCBI Official Symbol
Scg2
NCBI Official Synonym Symbols
Chgc; SgII
NCBI Protein Information
secretogranin-2; chromogranin-C
UniProt Protein Name
Secretogranin-2
Protein Family
UniProt Gene Name
Scg2
UniProt Synonym Gene Names
Chgc; Scg-2; SgII; SN
UniProt Entry Name
SCG2_MOUSE
Uniprot Description
SCG2: Secretogranin-2 is a neuroendocrine secretory granule protein, which is the precursor for biologically active peptides. Belongs to the chromogranin/secretogranin protein family.
Protein type: Motility/polarity/chemotaxis; Cell cycle regulation; Secreted, signal peptide; Apoptosis; Cytokine; Secreted
Cellular Component: extracellular space; extracellular region; intracellular; secretory granule
Molecular Function: cytokine activity; chemoattractant activity
Biological Process: positive chemotaxis; MAPKKK cascade; eosinophil chemotaxis; positive regulation of endothelial cell proliferation; angiogenesis; induction of positive chemotaxis
Research Articles on SCG2
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Product Notes
The SCG2 scg2 (Catalog #AAA2018496) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Secretogranin II (SCG2) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the SCG2 scg2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below. MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGS-AVLLL IHLIF LISGAEAASF QRNQLLQKEP DLRLENVQKF PSPEMIRALE YIEKLRQQAH REESSPDYNP YQGVSVPLQL KENGEESHLA ESSRDALSED EWMRIILEAL RQAENEPPSA PKENKPYALN LEKNFPVDTP DDYETQQWPE RKLKHMRFPL MYEENSRENP FKRTNEI. It is sometimes possible for the material contained within the vial of "Secretogranin II (SCG2), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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