Mitogen Activated Protein Kinase Kinase Kinase Kinase 5 (MAP4K5) Recombinant Protein | MAP4K5 recombinant protein

Recombinant Mitogen Activated Protein Kinase Kinase Kinase Kinase 5 (MAP4K5)

Cat. Num. AAA2018489

• Gene Names: Map4k5; KHS; GCKR; MAPKKKK5; 4432415E19Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Mitogen Activated Protein Kinase Kinase Kinase Kinase 5 (MAP4K5); Recombinant Mitogen Activated Protein Kinase Kinase Kinase Kinase 5 (MAP4K5); MAP4K5 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- AD ILRRNPQHDY ELVQRVGSGT YGDVYKARNV HTGELAAVKI IKLEPGDDFS LIQQEIFMVK ECKHCNIVAY FGSYLSREKL WICMEYCGGG SLQDIYHVTG PLSEMQIAYV CRETLQGLAY LHTKGKMHRD IKGANILLTD HGDVKLADFG VAAKITATIA KRKSFIGTPY WMAPEVAAVE KNGGYNQLCDIWAVGITAIE LGELQPPMFD LHPMRALFLM S
• Sequence Length: 847

• Applicable Applications for MAP4K5 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Ala9~Ser231 (Accession # Q8BPM2) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Cytoplasm
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.9
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 77736537
• NCBI GeneID: 399510
• NCBI Accession #: NP_958927.2
• NCBI GenBank Nucleotide #: NM_201519.2
• UniProt Accession #: Q8BPM2; Q6PEQ2; Q8C3U5; Q8CGF3; Q99LM7; Q9CX73
• Molecular Weight: 28.7kDa
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase kinase 5
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase kinase 5
• NCBI Official Symbol: Map4k5
• NCBI Official Synonym Symbols: KHS; GCKR; MAPKKKK5; 4432415E19Rik
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase kinase 5; MEKKK 5; MEK kinase kinase 5; MAPK/ERK kinase kinase kinase 5
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase kinase 5
• Protein Family: Mitogen-activated protein kinase kinase kinase kinase
• UniProt Gene Name: Map4k5
• UniProt Synonym Gene Names: MEK kinase kinase 5; MEKKK 5
• UniProt Entry Name: M4K5_MOUSE

Uniprot Description

KHS1: a serine/threonine protein kinase of the STE20 family. Highly similar to yeast SPS1/STE20 kinase. Activates Jun kinase in mammalian cells, which suggested a role in stress response.

Protein type: Protein kinase, STE; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; KHS subfamily

Cellular Component: cytoplasm; plasma membrane

Molecular Function: transferase activity; protein serine/threonine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; ATP binding; protein kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: response to stress; phosphorylation; protein amino acid phosphorylation