Macrophage Erythroblast Attacher (MAEA) Recombinant Protein | MAEA recombinant protein

Recombinant Macrophage Erythroblast Attacher (MAEA)

Cat. Num. AAA2018269

• Gene Names: MAEA; EMP; EMLP; GID9; PIG5; HLC-10
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Macrophage Erythroblast Attacher (MAEA); Recombinant Macrophage Erythroblast Attacher (MAEA); MAEA recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- CGYYN TAVKLARQSG IEDLVNIEMF LTAKEVEESL ERRETATCLA WCHDNKSRLR KMKSCLEFSL RIQEFIELIR QNKRLDAVRH ARKHFSQAEG SQLDEVRQAM GMLAFPPDTH ISPYKDLLDP ARWRMLIQQF RYDNYRLHQL GNNSVFTLTL QAGLSAIKTP QCYKEDGSSK SPDCPVCSRS LNKLAQPLPMAHCANSRLVC KISGDVMNEN NPPMMLPNGY VYGYNSLLSI RQDDKVVCP
• Sequence Length: 396

• Applicable Applications for MAEA recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Cys136~Pro379 (Accession # Q7L5Y9) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Nucleus matrix. Cell membrane. Cytoplasm, cytoskeleton
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 8.8
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 62953131
• NCBI GeneID: 10296
• NCBI Accession #: NP_001017405.1
• NCBI GenBank Nucleotide #: NM_001017405.1
• UniProt Accession #: Q7L5Y9; O95285; Q5JB54; Q6ZRD6; Q9BQ11; Q9H9V6; Q9H9Z4; Q9NW84
• Molecular Weight: 31.7kDa
• NCBI Official Full Name: macrophage erythroblast attacher isoform 1
• NCBI Official Synonym Full Names: macrophage erythroblast attacher
• NCBI Official Symbol: MAEA
• NCBI Official Synonym Symbols: EMP; EMLP; GID9; PIG5; HLC-10
• NCBI Protein Information: macrophage erythroblast attacher; proliferation-inducing gene 5; lung cancer-related protein 10; erythroblast macrophage protein; GID complex subunit 9, FYV10 homolog; human lung cancer oncogene 10 protein; cell proliferation-inducing gene 5 protein
• UniProt Protein Name: Macrophage erythroblast attacher
• Protein Family: Macrophage erythroblast attacher
• UniProt Gene Name: MAEA
• UniProt Synonym Gene Names: EMP; HLC-10
• UniProt Entry Name: MAEA_HUMAN

NCBI Description

This gene encodes a protein that mediates the attachment of erythroblasts to macrophages. This attachment promotes terminal maturation and enucleation of erythroblasts, presumably by suppressing apoptosis. The encoded protein is an integral membrane protein with the N-terminus on the extracellular side and the C-terminus on the cytoplasmic side of the cell. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

MAEA: Plays a role in erythroblast enucleation and in the development of the mature macrophages. Mediates the attachment of erythroid cell to mature macrophages, in correlation with the presence of MAEA at cell surface of mature macrophages; This MAEA- mediated contact inhibits erythroid cells apoptosis. Participates to erythroblastic island formation, which is the functional unit of definitive erythropoiesis. Associates with F-actin to regulate actin distribution in erythroblasts and macrophages. May contribute to nuclear architecture and cells division events. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Actin-binding; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: nucleoplasm; cytoskeleton; nuclear matrix; integral to plasma membrane; cytoplasm; contractile ring; spindle; nucleus

Molecular Function: actin binding

Biological Process: negative regulation of myeloid cell apoptosis; erythrocyte maturation; cell division; regulation of mitotic cell cycle; enucleate erythrocyte development; cytoskeleton organization and biogenesis; cell adhesion; cell cycle