Phospholipase A2, Cytosolic (cPLA2) Recombinant Protein | cPLA2 recombinant protein

Recombinant Phospholipase A2, Cytosolic (cPLA2)

Cat. Num. AAA2018234

• Gene Names: PLA2G4A; PLA2G4; cPLA2-alpha
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Phospholipase A2; Cytosolic (cPLA2); Recombinant Phospholipase A2; cPLA2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-RPQRGVDL IISFDFSARP SDSSPPFKEL LLAEKWAKMN KLPFPKIDPY VFDREGLKEC YVFKPKNPDM EKDCPTIIHF VLANINFRKY RAPGVPRETE EEKEIADFDI FDDPESPFST FNFQYPNQAF KRLHDLMHFN TLNNIDVIKE AMVESIEYRR QNPSRCSVSL SNVEARRFFN KEFLSKPKA
• Sequence Length: 749

• Applicable Applications for cPLA2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Arg563~Ala749 (Accession # P47712) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 23943920
• NCBI GeneID: 5321
• NCBI Accession #: NP_077734.1
• NCBI GenBank Nucleotide #: NM_024420.2
• UniProt Accession #: P47712; Q29R80; B1AKG4
• Molecular Weight: 23.5kDa
• NCBI Official Full Name: cytosolic phospholipase A2
• NCBI Official Synonym Full Names: phospholipase A2, group IVA (cytosolic, calcium-dependent)
• NCBI Official Symbol: PLA2G4A
• NCBI Official Synonym Symbols: PLA2G4; cPLA2-alpha
• NCBI Protein Information: cytosolic phospholipase A2; cPLA2; lysophospholipase; phospholipase A2 group IVA; phosphatidylcholine 2-acylhydrolase; calcium-dependent phospholipid-binding protein
• UniProt Protein Name: Cytosolic phospholipase A2
• UniProt Gene Name: PLA2G4A
• UniProt Synonym Gene Names: CPLA2; PLA2G4; cPLA2
• UniProt Entry Name: PA24A_HUMAN

NCBI Description

This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Uniprot Description

cPLA2: a calcium-dependent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Arachidonic acid is a precursor for the eicosanoids that are involved in hemodynamic regulation, inflammatory responses, and other cellular processes. Promotes cerebellar long-term depression and motor learning. Translocates to the Golgi and endoplasmic reticulum in a calcium-dependent fashion. Translocation and activation of at the ER facilitates the process of ER stress. Regulates the biogenesis of lipid droplets, a process dependent on JNK and ceramide kinase. Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+. The N-terminal C2 domain, by its association with lipid membranes, mediates the regulation of CPLA2 by presenting the active site to its substrate in response to elevations of cytosolic Ca2+. Inhibited when in trimolecular complex with ANXA2 and S100A10. Phosphorylation of S727 relieves this inhibitory interaction, thus activating PLA2G4A.

Protein type: Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - ether lipid; Lipid Metabolism - linoleic acid; EC 3.1.1.4; EC 3.1.1.5; Lipid Metabolism - arachidonic acid; Phospholipase; Lipid Metabolism - glycerophospholipid

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; mitochondrial inner membrane; cytoplasmic membrane-bound vesicle; cytoplasm; cytosol

Molecular Function: phospholipase A2 activity; calcium-dependent phospholipase A2 activity; calcium-dependent phospholipid binding; calcium ion binding; lysophospholipase activity

Biological Process: icosanoid biosynthetic process; platelet activating factor biosynthetic process; platelet activation; phospholipid catabolic process; phospholipid metabolic process; icosanoid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; arachidonic acid metabolic process; arachidonic acid secretion; blood coagulation; regulation of cell proliferation