Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit Platelet Activating Factor Acetylhydrolase Ib3 (PAFAH1B3) Polyclonal Antibody | anti-PAFAH1B3 antibody
Polyclonal Antibody to Platelet Activating Factor Acetylhydrolase Ib3 (PAFAH1B3)
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot; Sample: Lane1: Jurkat cell lysate;Lane2: Hela cell lysate Primary Ab: 1ug/ml Rabbit Anti-Human PAFAH1B3 AntibodySecond Ab: 0.2?g/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Western Blot (WB)
(Western Blot; Sample: Lane1: Jurkat cell lysate;Lane2: Hela cell lysate Primary Ab: 1ug/ml Rabbit Anti-Human PAFAH1B3 AntibodySecond Ab: 0.2?g/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Spleen Tissue; Primary Ab: 20ug/ml Rabbit Anti-Human PAFAH1B3 AntibodySecond Ab: 2?g/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Spleen Tissue; Primary Ab: 20ug/ml Rabbit Anti-Human PAFAH1B3 AntibodySecond Ab: 2?g/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an acetylhydrolase that catalyzes the removal of an acetyl group from the glycerol backbone of platelet-activating factor. The encoded enzyme is a subunit of the platelet-activating factor acetylhydrolase isoform 1B complex, which consists of the catalytic beta and gamma subunits and the regulatory alpha subunit. This complex functions in brain development. A translocation between this gene on chromosome 19 and the CDC-like kinase 2 gene on chromosome 1 has been observed, and was associated with cognitive disability, ataxia, and atrophy of the brain. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2009]
Uniprot Description
PAFAH1B3: Inactivates paf by removing the acetyl group at the sn-2 position. This is a catalytic subunit. Plays an important role during the development of brain. Belongs to the 'GDSL' lipolytic enzyme family. Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily.
Protein type: EC 3.1.1.47; Lipase; Lipid Metabolism - ether lipid
Chromosomal Location of Human Ortholog: 19q13.2
Cellular Component: cytoplasm; cytosol; membrane
Molecular Function: 1-alkyl-2-acetylglycerophosphocholine esterase activity; identical protein binding; phospholipase A2 activity; platelet-activating factor acetyltransferase activity; protein binding; protein heterodimerization activity
Biological Process: brain development; lipid catabolic process; lipid metabolic process; nervous system development; spermatogenesis
Research Articles on PAFAH1B3
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Product Notes
The PAFAH1B3 pafah1b3 (Catalog #AAA2016988) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Platelet Activating Factor Acetylhydrolase Ib3 (PAFAH1B3) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Platelet Activating Factor Acetylhydrolase Ib3 (PAFAH1B3) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the PAFAH1B3 pafah1b3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Platelet Activating Factor Acetylhydrolase Ib3 (PAFAH1B3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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