Rabbit anti-Human Kallikrein 11 (KLK11) Polyclonal Antibody | anti-KLK11 antibody

Polyclonal Antibody to Kallikrein 11 (KLK11)

Cat. Num. AAA2015878

• Gene Names: KLK11; TLSP; PRSS20
• Reactivity: Human
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Kallikrein 11 (KLK11); Polyclonal Antibody; Polyclonal Antibody to Kallikrein 11 (KLK11); anti-KLK11 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against KLK11. It has been selected for its ability to recognize KLK11 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence: Antigen: The target protein is fused with N-terminal Tags, His-tag and T7-tag and its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- IL LALATGLVGG ETRIIKGFEC KPHSQPWQAA LFEKTRLLCG ATLIAPRWLL TAAHCLKPRY IVHLGQHNLQ KEEGCEQTRT ATESFPHPGF NNSLPNKDHR NDIMLVKMAS PVSITWAVRP LTLSSRCVTA GTSCLISGWG STSSPQLRLP HTLRCANITI IEHQKCENAY PGNITDTMVC ASVQEGGKDS CQGDSGGPLV
• Sequence Length: 275

• Applicable Applications for anti-KLK11 antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant KLK11 (Ile39~Asn282) expressed in E.coli.
• Cross Reactivity: Human, Mouse
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2045763
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Lane1: Mouse Thymus Tissue; Lane2: Mouse Skin Tissue; Lane3: Human K562 Cells.)

Western Blot (WB)

(Western Blot: Sample: Recombinant KLK11, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Stomach Cancer Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Prostate Gland Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Kidney Tissue)

Knockout Validation

(Knockout Validation: Lane 1: Wild-type K562 cell lysate; Lane 2: KLK11 knockout K562 cell lysate; Predicted MW: 30kd Observed MW: 40kd Primary Ab: 2ug/ml Rabbit Anti-Human KLK11 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))

Product Categories/Family for anti-KLK11 antibody

Knock-Out Validation Antibody

NCBI and Uniprot Product Information

• NCBI GI #: 209862865
• NCBI GeneID: 11012
• NCBI Accession #: NP_001129504.1
• NCBI GenBank Nucleotide #: NM_001136032.2
• UniProt Accession #: Q9UBX7; O75837; Q0WXX5; Q8IXD7; Q9NS65
• Molecular Weight: 30,166 Da
• NCBI Official Full Name: kallikrein-11 isoform 1
• NCBI Official Synonym Full Names: kallikrein related peptidase 11
• NCBI Official Symbol: KLK11
• NCBI Official Synonym Symbols: TLSP; PRSS20
• NCBI Protein Information: kallikrein-11
• UniProt Protein Name: Kallikrein-11
• Protein Family: Kallikrein
• UniProt Gene Name: KLK11
• UniProt Synonym Gene Names: PRSS20; TLSP; hK11

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing and the use of alternate promoters results in multiple transcript variants encoding distinct isoforms which are differentially expressed. [provided by RefSeq, Dec 2016]

Uniprot Description

KLK11: Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues. Belongs to the peptidase S1 family. Kallikrein subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.-; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: extracellular space; Golgi apparatus

Molecular Function: serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: proteolysis