Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Human Galectin 12 (GAL12) Polyclonal Antibody | anti-GAL12 antibody
Polyclonal Antibody to Galectin 12 (GAL12)
Gene Names
LGALS12; GAL12; GRIP1
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Galectin 12 (GAL12); Polyclonal Antibody; Polyclonal Antibody to Galectin 12 (GAL12); anti-GAL12 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against GAL12. It has been selected for its ability to recognize GAL12 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- P LDAHRFQVDF QCGCSLCPRP DIAFHFNPRF HTTKPHVICN TLHGGRWQRE ARWPHLALRR GSSFLILFLF GNEEVKVSVN GQHFLHFRYR LPLSHVDTLG IFGDILVEAV GFLNINPFVE GSREYPAGHP FLLMSPRLEV PCSHALPQGL SPGQVIIVRG LVLQEPKHFT VSLRDQAAHA PVTLRASFAD
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- P LDAHRFQVDF QCGCSLCPRP DIAFHFNPRF HTTKPHVICN TLHGGRWQRE ARWPHLALRR GSSFLILFLF GNEEVKVSVN GQHFLHFRYR LPLSHVDTLG IFGDILVEAV GFLNINPFVE GSREYPAGHP FLLMSPRLEV PCSHALPQGL SPGQVIIVRG LVLQEPKHFT VSLRDQAAHA PVTLRASFAD
Sequence Length
266
Applicable Applications for anti-GAL12 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant GAL12 (Pro70~Leu291) expressed in E.coli.
Cross Reactivity
Human
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2040978
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,629 Da
NCBI Official Full Name
galectin-12 isoform 1
NCBI Official Synonym Full Names
galectin 12
NCBI Official Symbol
LGALS12
NCBI Official Synonym Symbols
GAL12; GRIP1
NCBI Protein Information
galectin-12
UniProt Protein Name
Galectin-12
Protein Family
UniProt Gene Name
LGALS12
UniProt Synonym Gene Names
GRIP1; Gal-12
NCBI Description
This gene encodes a member of the galectin superfamily, a group of beta-galactoside-binding proteins with conserved carbohydrate recognition domains. The related mouse protein is a primary regulator of the early stages of adipose tissue development. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, May 2010]
Uniprot Description
LGALS12: Binds lactose. May participate in the apoptosis of adipocytes. 6 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 11q12.3
Cellular Component: mitochondrion; nucleus
Molecular Function: lactose binding
Biological Process: regulation of fat cell differentiation; regulation of lipid catabolic process
Research Articles on GAL12
Similar Products
Product Notes
The GAL12 lgals12 (Catalog #AAA2013981) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Galectin 12 (GAL12) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Galectin 12 (GAL12) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the GAL12 lgals12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- P LDAHRFQVDF QCGCSLCPRP DIAFHFNPRF HTTKPHVICN TLHGGRWQRE ARWPHLALRR GSSFLILFLF GNEEVKVSVN GQHFLHFRYR LPLSHVDTLG IFGDILVEAV GFLNINPFVE GSREYPAGHP FLLMSPRLEV PCSHALPQGL SPGQVIIVRG LVLQEPKHFT VSLRDQAAHA PVTLRASFAD. It is sometimes possible for the material contained within the vial of "Galectin 12 (GAL12), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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