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Western Blot (WB) (Western Blot: Sample: Lane1: Mouse Heart Tissue; Lane2: Mouse Brain Tissue.)

Rabbit anti-Human Tubulin Polymerization Promoting Protein (TPPP) Polyclonal Antibody | anti-TPPP antibody

Polyclonal Antibody to Tubulin Polymerization Promoting Protein (TPPP)

Gene Names
TPPP; p24; p25; TPPP1; TPPP/p25; p25alpha
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Tubulin Polymerization Promoting Protein (TPPP); Polyclonal Antibody; Polyclonal Antibody to Tubulin Polymerization Promoting Protein (TPPP); anti-TPPP antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against TPPP. It has been selected for its ability to recognize TPPP in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- AKA ANRTPPKSPG DPSKDRAAKR LSLESEGAGE GAAASPELSA LEEAFRRFAV HGDARATGRE MHGKNWSKLC KDCQVIDGRN VTVTDVDIVF SKIKGKSCRT ITFEQFQEAL EELAKKRFKD KSSEEAVREV HRLIEGKAPI ISGVTKAISS PTVSRLTDTT KFTGSHKERF DPSGKGKGKA
Sequence Length
219
Applicable Applications for anti-TPPP antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant TPPP (Ala8~Thr210) expressed in E.coli.
Cross Reactivity
Human, Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2050327
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Lane1: Mouse Heart Tissue; Lane2: Mouse Brain Tissue.)

Western Blot (WB) (Western Blot: Sample: Lane1: Mouse Heart Tissue; Lane2: Mouse Brain Tissue.)

Western Blot (WB)

(Western Blot: Sample: Recombinant TPPP, Human.)

Western Blot (WB) (Western Blot: Sample: Recombinant TPPP, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Stomach Cancer Tissue.)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Stomach Cancer Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Brain Tissue)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Brain Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Stomach Tissue)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Stomach Tissue)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
23,694 Da
NCBI Official Full Name
tubulin polymerization-promoting protein
NCBI Official Synonym Full Names
tubulin polymerization promoting protein
NCBI Official Symbol
TPPP
NCBI Official Synonym Symbols
p24; p25; TPPP1; TPPP/p25; p25alpha
NCBI Protein Information
tubulin polymerization-promoting protein
UniProt Protein Name
Tubulin polymerization-promoting protein
UniProt Gene Name
TPPP
UniProt Synonym Gene Names
TPPP1; TPPP

Uniprot Description

TPPP: May play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. May play a role in mitotic spindle assembly and nuclear envelope breakdown. Belongs to the TPPP family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 5p15.33

Cellular Component: cytoplasm; cytosol; microtubule; mitochondrion; myelin sheath; nucleus; perinuclear region of cytoplasm

Molecular Function: microtubule binding; protein binding; tubulin binding

Biological Process: microtubule bundle formation; microtubule polymerization; positive regulation of protein complex assembly; positive regulation of protein polymerization

Research Articles on TPPP

Similar Products

Product Notes

The TPPP tppp (Catalog #AAA2013350) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Tubulin Polymerization Promoting Protein (TPPP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Tubulin Polymerization Promoting Protein (TPPP) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the TPPP tppp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- AKA ANRTPPKSPG DPSKDRAAKR LSLESEGAGE GAAASPELSA LEEAFRRFAV HGDARATGRE MHGKNWSKLC KDCQVIDGRN VTVTDVDIVF SKIKGKSCRT ITFEQFQEAL EELAKKRFKD KSSEEAVREV HRLIEGKAPI ISGVTKAISS PTVSRLTDTT KFTGSHKERF DPSGKGKGKA. It is sometimes possible for the material contained within the vial of "Tubulin Polymerization Promoting Protein (TPPP), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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