Relaxin/Insulin Like Family Peptide Receptor 1 (RXFP1) Recombinant Protein | RXFP1 recombinant protein

Recombinant Relaxin/Insulin Like Family Peptide Receptor 1 (RXFP1)

Cat. Num. AAA2012340

• Gene Names: RXFP1; LGR7; RXFPR1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Relaxin/Insulin Like Family Peptide Receptor 1 (RXFP1); Recombinant Relaxin/Insulin Like Family Peptide Receptor 1 (RXFP1); RXFP1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG SEF-P DCFKNYHDLQ KLYLQNNKIT SISIYAFRGL NSLTKLYLSH NRITFLKPGV FEDLHRLEWL IIEDNHLSRISPPTFYGLNS LILLVLMNNV LTRLPDKPLC QHMPRLHWLD LEGNHIHNLR NLTFISCSNL TVLVMRKNKI NHLNENTFA
• Sequence Length: 724

• Applicable Applications for RXFP1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Pro110~Ala259 (Accession # Q9HBX9) with N-terminal His-Tag
• Subcellular Location: Cell membrane; Multi-pass membrane protein.
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 19.2kDa
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 359279870
• NCBI GeneID: 59350
• NCBI Accession #: NP_001240657.1
• NCBI GenBank Nucleotide #: NM_001253728.1
• UniProt Accession #: Q9HBX9; Q2M215; Q3KU24; Q3KU25; Q3KU26; B4DTV2
• Molecular Weight: 19.2kDa
• NCBI Official Full Name: relaxin receptor 1 isoform 3
• NCBI Official Synonym Full Names: relaxin/insulin-like family peptide receptor 1
• NCBI Official Symbol: RXFP1
• NCBI Official Synonym Symbols: LGR7; RXFPR1
• NCBI Protein Information: relaxin receptor 1; leucine-rich repeat-containing G protein-coupled receptor 7
• UniProt Protein Name: Relaxin receptor 1
• Protein Family: Relaxin receptor
• UniProt Gene Name: RXFP1
• UniProt Synonym Gene Names: LGR7
• UniProt Entry Name: RXFP1_HUMAN

NCBI Description

This gene encodes a member of the leucine-rich repeat-containing subgroup of the G protein-coupled 7-transmembrane receptor superfamily. The encoded protein plays a critical role in sperm motility, pregnancy and parturition as a receptor for the protein hormone relaxin. Decreased expression of this gene may play a role in endometriosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

RXFP1: Receptor for relaxins. The activity of this receptor is mediated by G proteins leading to stimulation of adenylate cyclase and an increase of cAMP. Binding of the ligand may also activate a tyrosine kinase pathway that inhibits the activity of a phosphodiesterase that degrades cAMP. Belongs to the G-protein coupled receptor 1 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q32.1

Cellular Component: integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; protein binding; metal ion binding

Biological Process: G-protein coupled receptor protein signaling pathway