Immunocytochemistry (ICC)/Immunofluorescence (IF)
(ICC/IF analysis of AGXT in Hep3B cells. The cell was stained at GA0580 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)
Mouse anti-Human AGXT Monoclonal Antibody | anti-AGXT antibody
AGXT
Gene Names
AGXT; AGT; PH1; SPT; AGT1; SPAT; TLH6; AGXT1
Reactivity
Human
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
By protein-A affinity chromatography
Synonyms
AGXT; Monoclonal Antibody; Serpin B5; Protease inhibitor 5; SERPINB5; alanine-glyoxylate aminotransferase; AGXT1; PH1; AGT; SPT; AGT1; oxalosis I; primary hyperoxaluria type 1; L-alanine: glyoxylate aminotransferase 1; serine:pyruvate aminotransferase; glycolicaciduria; anti-AGXT antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b kappa
Clone Number
AT2T4
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
392
Applicable Applications for anti-AGXT antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity.
Antigen Species
Human
Immunogen
Recombinant AGXT (330-392aa) purified from E. coli
Conjugation
Unconjugated
Note
This product was produced from tissue culture supernatant.
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
Immunocytochemistry (ICC)/Immunofluorescence (IF)
(ICC/IF analysis of AGXT in Hep3B cells. The cell was stained at GA0580 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)
Immunocytochemistry (ICC)/Immunofluorescence (IF)
(ICC/IF analysis of AGXT in Hep3B cells. The cell was stained at GA0580 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)
Related Product Information for anti-AGXT antibody
The AGXT gene provides instructions for making a liver enzyme called alanine-glyoxylate aminotransferase.Inside liver cells, this enzyme is found in peroxisomes, structures that contain many different enzymes used toproduce energy and the basic materials important for cellular activities. AGXT converts a compound calledglyoxylate to glycine, an amino acid that is a building block for making enzymes and other proteins.
Product Categories/Family for anti-AGXT antibody
References
Donini S, et al., (2009) Biochem J 422(2):265-272.; Grujic D, et al., (2009) Am J Nephrol 29(2):86-93.; Williams EL, et al., (2009) Hum Mutat 30(6):910-917.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
serine--pyruvate aminotransferase
NCBI Official Synonym Full Names
alanine--glyoxylate and serine--pyruvate aminotransferase
NCBI Official Symbol
AGXT
NCBI Official Synonym Symbols
AGT; PH1; SPT; AGT1; SPAT; TLH6; AGXT1
NCBI Protein Information
serine--pyruvate aminotransferase
UniProt Protein Name
Serine--pyruvate aminotransferase
Protein Family
UniProt Gene Name
AGXT
UniProt Synonym Gene Names
AGT1; SPAT; SPT; AGT
NCBI Description
This gene is expressed only in the liver and the encoded protein is localized mostly in the peroxisomes, where it is involved in glyoxylate detoxification. Mutations in this gene, some of which alter subcellular targetting, have been associated with type I primary hyperoxaluria. [provided by RefSeq, Jul 2008]
Research Articles on AGXT
Similar Products
Product Notes
The AGXT agxt (Catalog #AAA201222) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AGXT reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AGXT can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Researchers should empirically determine the suitability of the AGXT agxt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AGXT, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
