Tenascin C (TNC) Recombinant Protein | TNC recombinant protein

Recombinant Tenascin C (TNC)

Cat. Num. AAA2012158

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Tenascin C (TNC); Recombinant Tenascin C (TNC); TNC recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-DGQCICED GFTGSDCAEL SCPGDCHGHG RCVNGQCICH EGFTGXDCKE QRCPSDCHGQ GRCEDGQCIC HEGFTGLDCG QRSCPNDCSN QGQCVAGRCI CNEGYTGVDC S
• Sequence Length: 2019

• Applicable Applications for TNC recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Asp513~Ser621 (Accession # B2LYI9) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 198278535
• NCBI GeneID: 116640
• NCBI Accession #: NP_446313.1
• NCBI GenBank Nucleotide #: NM_053861.1
• UniProt Accession #: B2LYI9
• Molecular Weight: 13.0kDa
• NCBI Official Full Name: tenascin C
• NCBI Official Synonym Full Names: tenascin C
• NCBI Official Symbol: Tnc
• NCBI Protein Information: tenascin C
• UniProt Protein Name: Tenascin C
• Protein Family: Tenascin
• UniProt Gene Name: Tnc
• UniProt Entry Name: B2LYI9_RAT

NCBI Description

extracellular matrix glycoprotein; inhibits the attachment of fibroblasts to adhesive proteins [RGD, Feb 2006]

Uniprot Description

TNC: Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. Belongs to the tenascin family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; focal adhesion; membrane; extracellular region; basement membrane

Molecular Function: syndecan binding

Biological Process: osteoblast differentiation; response to ethanol; wound healing; response to mechanical stimulus; positive regulation of cell proliferation; response to wounding; negative regulation of cell adhesion; axon regeneration in the peripheral nervous system; neuromuscular junction development; neurite development; odontogenesis of dentine-containing teeth