RAGE active protein

RAGE

Cat. Num. AAA201188

• Gene Names: AGER; RAGE; SCARJ1
• Applications: SDS-Page
• Purity: >90% by SDS - PAGE
• Synonyms: RAGE; AGER; advanced glycosylation end product-specific receptor isoform 1; SCARJ1; Receptor for advanced glycosylation end products; RAGE active protein

• Host: Baculovirus
• Purity/Purification: >90% by SDS - PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
• Concentration: 0.25mg/ml (determined by BCA assay) (varies by lot)
• Sequence Positions: 24-342aa
• Sequence: QNITARI GEPLVLKCKG APKKPPQRLE WKLNTGRTEA WKVLSPQGGG PWDSVARVLP NGSLFLPAVG IQDEGIFRCQ AMNRNGKETK SNYRVRVYQI PGKPEIVDSA SELTAGVPNK VGTCVSEGSY PAGTLSWHLD GKPLVPNEKG VSVKEQTRRH PETGLFTLQS ELMVTPARGG DPRPTFSCSF SPGLPRHRAL RTAPIQPRVW EPVPLEEVQL VVEPEGGAVA PGGTVTLTCE VPAQPSPQIH WMKDGVPLPL PPSPVLILPE IGPQDQGTYS CVATHSSHGP QESRAVSISI IEPGEEGPTA GSVGGSGLGT LA
• Sequence Length: 404

• Applicable Applications for RAGE active protein: SDS-PAGE, Bioactivity
• Antigen Species: Human
• Tag: hIgG-His-Tag
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Predicted N-Terminal: Ala
• Biological activity: Measured by the ability of the binding activity in a functional ELISA. The ED50 range <= 15 ug/ml.
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for RAGE active protein

RAGE, also known as advanced glycosylation end product-specific receptor isoform 1, is a member of the immunoglobulin super-family transmembrane proteins. It mediates interactions of advanced glycosylation end products (AGE). AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. This protein contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. Recombinant human RAGE, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for RAGE active protein

Neuroscience

References

Giurdanella G., et al. (2017) Biochem Pharmacol. 142:145-154; Zhang L., et al. (2019) Int J Mol Med. 44:885-892

NCBI and Uniprot Product Information

• NCBI GI #: 10835203
• NCBI GeneID: 177
• NCBI Accession #: NP_001127
• NCBI GenBank Nucleotide #: NP_001127.1
• UniProt Accession #: Q15109
• Molecular Weight: 61.2kDa (561aa)
• NCBI Official Full Name: advanced glycosylation end product-specific receptor isoform 1
• NCBI Official Synonym Full Names: advanced glycosylation end-product specific receptor
• NCBI Official Symbol: AGER
• NCBI Official Synonym Symbols: RAGE; SCARJ1
• NCBI Protein Information: advanced glycosylation end product-specific receptor
• UniProt Protein Name: Advanced glycosylation end product-specific receptor
• UniProt Gene Name: AGER
• UniProt Synonym Gene Names: RAGE
• UniProt Entry Name: RAGE_HUMAN

NCBI Description

The advanced glycosylation end product (AGE) receptor encoded by this gene is a member of the immunoglobulin superfamily of cell surface receptors. It is a multiligand receptor, and besides AGE, interacts with other molecules implicated in homeostasis, development, and inflammation, and certain diseases, such as diabetes and Alzheimer's disease. Many alternatively spliced transcript variants encoding different isoforms, as well as non-protein-coding variants, have been described for this gene (PMID:18089847). [provided by RefSeq, May 2011]

Uniprot Description

RAGE: Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF- alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Interacts with S100B, S100A1 and APP. Interacts with S100A12. Endothelial cells. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Motility/polarity/chemotaxis; Cell cycle regulation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: integral to plasma membrane; extracellular region; plasma membrane

Molecular Function: identical protein binding; protein binding; transmembrane receptor activity; receptor activity

Biological Process: cell surface receptor linked signal transduction; response to wounding; innate immune response; inflammatory response; neurite development; induction of positive chemotaxis; activation of NF-kappaB transcription factor