Aldehyde Dehydrogenase 7 Family, Member A1 (ALDH7A1) Recombinant Protein | ALDH7A1 recombinant protein

Recombinant Aldehyde Dehydrogenase 7 Family, Member A1 (ALDH7A1)

Cat. Num. AAA2011567

• Gene Names: Aldh7a1; antiquitin
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Aldehyde Dehydrogenase 7 Family; Member A1 (ALDH7A1); Recombinant Aldehyde Dehydrogenase 7 Family; ALDH7A1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGS-LMS TLLIHHPQYA WLQDLGLRED NEGVFNGSWG GRGEVITTYC PANNEPIARV RQASMKDYEE TIGKAKKAWN IWADIPAPKR GEIVRKIGDA LREKIQLLGR LVSLEMGKIL VEGIGEVQEY VDVCDYAAGL SRMIGGPTLP SERPGHALME QWNPLGLVGI ITAFNFPVAV FGWNNAIALI TGNVCLWKGA PTTSLVSIAV TKIIAKVLED NLLPGAICSL TCGGADMGTA MARDERVNLL SFTGSTQVGK QVALMVQERF GKSLLELGGN NAIIAFEDAD LSLVLPSALF AAVGTAGQRC TTVRRLFLHE SIHDEVVDRL KNAYSQIRVG NPWDPNILYG PLHTKQAVSM FVQAVEEAKK EGGTVVYGGK VMDHPGNYVE PTIVTGLVHD APIVHKETFA PILYVFKFKN EEEVFEWNNE VKQGLSSSIF TKDLGRIFRW LGPKGSDCGI VNVNIPTSGA EIGGAFGGEK HTGGGRESGS DAWKQYMRRS TCTINYSTAL PLAQGIKFQ
• Sequence Length: 539

• Applicable Applications for ALDH7A1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Leu28~Gln539 (Accession # Q64057) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 402766107
• NCBI GeneID: 291450
• NCBI Accession #: NP_001258034.1
• NCBI GenBank Nucleotide #: NM_001271105.1
• UniProt Accession #: Q64057
• Molecular Weight: 57.0kDa
• NCBI Official Full Name: alpha-aminoadipic semialdehyde dehydrogenase
• NCBI Official Synonym Full Names: aldehyde dehydrogenase 7 family, member A1
• NCBI Official Symbol: Aldh7a1
• NCBI Official Synonym Symbols: antiquitin
• NCBI Protein Information: alpha-aminoadipic semialdehyde dehydrogenase; antiquitin-1; P6c dehydrogenase; alpha-AASA dehydrogenase; betaine aldehyde dehydrogenase; aldehyde dehydrogenase family 7 member A1; aldehyde dehydrogenase family 7, member A1; delta1-piperideine-6-carboxylate dehydrogenase; delta1-piperideine-6-carboxylate dehydrogenease
• UniProt Protein Name: Alpha-aminoadipic semialdehyde dehydrogenase
• Protein Family: Alpha-aminoadipic semialdehyde dehydrogenase
• UniProt Gene Name: Aldh7a1
• UniProt Synonym Gene Names: Ald7a1; Alpha-AASA dehydrogenase; P6c dehydrogenase
• UniProt Entry Name: AL7A1_RAT

Uniprot Description

ALDH7A1: Multifunctional enzyme mediating important protective effects. Metabolizes betaine aldehyde to betaine, an important cellular osmolyte and methyl donor. Protects cells from oxidative stress by metabolizing a number of lipid peroxidation-derived aldehydes. Involved in lysine catabolism. Defects in ALDH7A1 are the cause of pyridoxine-dependent epilepsy (PDE). PDE is characterized by a combination of various seizure types. It usually occurs in the first hours of life and is unresponsive to standard anticonvulsants, responding only to immediate administration of pyridoxine hydrochloride. Belongs to the aldehyde dehydrogenase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - butanoate; Amino Acid Metabolism - lysine degradation; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - propanoate; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Carbohydrate Metabolism - ascorbate and aldarate; Lipid Metabolism - glycerolipid; EC 1.2.1.3; Mitochondrial; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - tryptophan; Secondary Metabolites Metabolism - limonene and pinene degradation; Other Amino Acids Metabolism - beta-alanine; EC 1.2.1.31; Amino Acid Metabolism - histidine; Oxidoreductase; EC 1.2.1.8; Lipid Metabolism - fatty acid

Cellular Component: mitochondrion; cytoplasm; cytosol; nucleus

Molecular Function: aldehyde dehydrogenase (NAD) activity; L-aminoadipate-semialdehyde dehydrogenase activity; betaine-aldehyde dehydrogenase activity

Biological Process: glycine betaine biosynthetic process from choline