Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2) Recombinant Protein | ENPP2 recombinant protein

Recombinant Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2)

Cat. Num. AAA2011514

• Gene Names: ENPP2; ATX; NPP2; ATX-X; PDNP2; LysoPLD; AUTOTAXIN; PD-IALPHA
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2); Recombinant Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2); ENPP2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-RLHT KGSTEERHLL YGRPAVLYRT RYDILYHTDF ESGYSEIFLM PLWTSYTVSK QAEVSSVPDH LTSCVRPDVR VSPSFSQNCL AYKNDKQMSY GFLFPPYLSS SPEAKYDAFL VTNMVPMYPA FKRVWNYFQR VLVKKYASER NGVNVISGPI FDYDYDGLHD TEDKIKQYVE GSSIPVPTHY YSIITSCLDF TQPADKCDGP LSVSSFILPH RPDNEESCNS SEDESKWVEE LMKMHTARVR DIEHLTSLDF FRKTSRSYPE ILTLK
• Sequence Length: 863

• Applicable Applications for ENPP2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP); (May be suitable for use in other assays to be determined by the end user.)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Arg637~Lys905 (Accession # Q13822) with two N-terminal Tags, His-tag and T7-tag
• Predicted isoelectric point: 6.5
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• USAGE: Reconstitute in ddH2O.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 91823602
• NCBI GeneID: 5168
• NCBI Accession #: NP_001035181.1
• NCBI GenBank Nucleotide #: NM_001040092.2
• UniProt Accession #: Q13822; Q13827; Q14555; Q15117; Q9UCQ8; Q9UCR0; Q9UCR1; Q9UCR2; Q9UCR3; Q9UCR4; A8UHA1; E9PHP7
• Molecular Weight: 35.0kDa
• NCBI Official Full Name: ectonucleotide pyrophosphatase/phosphodiesterase family member 2 isoform 2 preproprotein
• NCBI Official Synonym Full Names: ectonucleotide pyrophosphatase/phosphodiesterase 2
• NCBI Official Symbol: ENPP2
• NCBI Official Synonym Symbols: ATX; NPP2; ATX-X; PDNP2; LysoPLD; AUTOTAXIN; PD-IALPHA
• NCBI Protein Information: ectonucleotide pyrophosphatase/phosphodiesterase family member 2; E-NPP 2; autotaxin-t; plasma lysophospholipase D; extracellular lysophospholipase D; phosphodiesterase I/nucleotide pyrophosphatase 2
• UniProt Protein Name: Ectonucleotide pyrophosphatase/phosphodiesterase family member 2
• Protein Family: Ectonucleotide pyrophosphatase/phosphodiesterase family
• UniProt Gene Name: ENPP2
• UniProt Synonym Gene Names: ATX; PDNP2; E-NPP 2; LysoPLD
• UniProt Entry Name: ENPP2_HUMAN

NCBI Description

The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2008]

Uniprot Description

ENPP2: Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility- related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Phosphodiesterase; Lipid Metabolism - ether lipid; Secreted, signal peptide; EC 3.1.4.39; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 8q24.1

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane

Molecular Function: phosphodiesterase I activity; nucleotide diphosphatase activity; nucleic acid binding; zinc ion binding; hydrolase activity; calcium ion binding; transcription factor binding; scavenger receptor activity; alkylglycerophosphoethanolamine phosphodiesterase activity; polysaccharide binding; lysophospholipase activity

Biological Process: receptor-mediated endocytosis; G-protein coupled receptor protein signaling pathway; phospholipid catabolic process; immune response; chemotaxis; cell motility; regulation of cell migration; regulation of angiogenesis; phosphate metabolic process