FK506 Binding Protein 1A (FKBP1A) Recombinant Protein | FKBP1A recombinant protein

Recombinant FK506 Binding Protein 1A (FKBP1A)

Cat. Num. AAA2011397

• Gene Names: FKBP1A; FKBP1; PKC12; PKCI2; FKBP12; PPIASE; FKBP-12; FKBP-1A
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: FK506 Binding Protein 1A (FKBP1A); Recombinant FK506 Binding Protein 1A (FKBP1A); FKBP1A recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-GVQVETISP GDGRTFPKRG QTCVVHYTGM LEDGKKFDSS RDRNKPFKFM LGKQEVIRGW EEGVAQMSVG QRAKLTISPD YAYGATGHPG IIPPHATLVF DVELLKLE
• Sequence Length: 108

• Applicable Applications for FKBP1A recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Gly2~Glu108 (Accession # P62942) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 4503725
• NCBI GeneID: 2280
• NCBI Accession #: NP_000792.1
• NCBI GenBank Nucleotide #: NM_000801.4
• UniProt Accession #: P62942; P20071; Q4VC47; Q6FGD9; Q6LEU3; Q9H103; Q9H566; D3DVW6
• Molecular Weight: 13.3kDa
• NCBI Official Full Name: peptidyl-prolyl cis-trans isomerase FKBP1A isoform a
• NCBI Official Synonym Full Names: FK506 binding protein 1A, 12kDa
• NCBI Official Symbol: FKBP1A
• NCBI Official Synonym Symbols: FKBP1; PKC12; PKCI2; FKBP12; PPIASE; FKBP-12; FKBP-1A
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase FKBP1A; rotamase; 12 kDa FKBP; calstabin 1; calstabin-1; FKBP12-Exip3; PPIase FKBP1A; immunophilin FKBP12; FK506 binding protein12; FK506-binding protein 1; FK506-binding protein 12; 12 kDa FK506-binding protein; protein kinase C inhibitor 2; FK506-binding protein 1A (12kD); FK506-binding protein, T-cell, 12-kD
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase FKBP1A
• Protein Family: Peptidyl-prolyl cis-trans isomerase
• UniProt Gene Name: FKBP1A
• UniProt Synonym Gene Names: FKBP1; FKBP12; PPIase FKBP1A; 12 kDa FKBP; FKBP-12; FKBP-1A
• UniProt Entry Name: FKB1A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. The protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. It also interacts with multiple intracellular calcium release channels, and coordinates multi-protein complex formation of the tetrameric skeletal muscle ryanodine receptor. In mouse, deletion of this homologous gene causes congenital heart disorder known as noncompaction of left ventricular myocardium. Multiple alternatively spliced variants, encoding the same protein, have been identified. The human genome contains five pseudogenes related to this gene, at least one of which is transcribed. [provided by RefSeq, Sep 2008]

Uniprot Description

FKBP12: an immunophilin and peptidyl-prolyl cis-trans isomerase that binds to the immunosuppressant drugs FK506 (a.k.a. tacrolimus or Fujimycin) and rapamycin (a.k.a. Sirolimus). The FKBP12/rapamycin complex binds NFAT transcription factors and inhibits the induction of T cell genes including IL-2, IL-3, IL-4, TNF-alpha and GM-CSF. FK506 is used in treating patients after organ transplant and those suffering from autoimmune disorders. The immunosuppressant activity of FKBP12 is not apparently related to its prolyl isomerase activity. The FKBP12/rapamycin complex inhibits the mammalian target of rapamycin (mTOR) pathway by directly binding the mTOR Complex1 (mTORC1). mTORC1 contains Raptor, mLST8, and PRAS40, and interacts with DEPTOR, which inhibits its activity. mTOR, a S/T protein kinase, is a central regulator of cellular growth and metabolism. Its inhibition enhances the dependence on aerobic glycolysis in leukemic cells.

Protein type: EC 5.2.1.8; Isomerase

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: endoplasmic reticulum membrane; sarcoplasmic reticulum membrane; membrane; cytoplasm; nerve terminal; cytosol; Z disc

Molecular Function: signal transducer activity; protein binding; FK506 binding; peptidyl-prolyl cis-trans isomerase activity; enzyme binding; protein homodimerization activity; macrolide binding; activin binding; Hsp70 protein binding; calcium channel inhibitor activity; SMAD binding; transforming growth factor beta receptor binding

Biological Process: heart morphogenesis; protein maturation via protein folding; protein peptidyl-prolyl isomerization; positive regulation of protein binding; protein folding; T cell activation; response to caffeine; T cell proliferation; regulation of protein localization; muscle contraction; negative regulation of protein amino acid phosphorylation; negative regulation of release of sequestered calcium ion into cytosol; transforming growth factor beta receptor signaling pathway; fibril organization and biogenesis; ventricular cardiac muscle morphogenesis; protein refolding; response to iron ion; regulation of immune response; positive regulation of I-kappaB kinase/NF-kappaB cascade; cytokine and chemokine mediated signaling pathway; regulation of activin receptor signaling pathway; SMAD protein complex assembly; negative regulation of protein phosphatase type 2B activity; 'de novo' protein folding; positive regulation of protein ubiquitination; release of sequestered calcium ion into cytosol