Surfactant Associated Protein D (SPD) Recombinant Protein | SPD recombinant protein

Recombinant Surfactant Associated Protein D (SPD)

Cat. Num. AAA2011246

• Gene Names: SFTPD; SP-D; PSP-D; SFTP4; COLEC7
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Surfactant Associated Protein D (SPD); Recombinant Surfactant Associated Protein D (SPD); SPD recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-AEMKTYSHRT MPSACTLVMC SSVESGLPGR DGRDGREGPR GEKGDPGLPG AAGQAGMPGQ AGPVGPKGDN GSVGEPGPKG DTGPSGPPGP PGVPGPAGRE GPLGKQGNIG PQGKPGPKGE AGPKGEVGAP GMQGSAGARG LAGPKGERGV PGERGVPGNT GAAGSAGAMG PQGSPGARGP PGLKGDKGIP GDKGAKGESG LPDVASLRQQ VEALQGQVQH LQAAFSQYKK VELFPNGQSV GEKIFKTAGF VKPFTEAQLL CTQAGGQLAS PRSAAENAAL QQLVVAKNEA AFLSMTDSKT EGKFTYPTGE SLVYSNWAPG EPNDDGGSED CVEIFTNGKW NDRACGEKRL VVCEF
• Sequence Length: 375

• Applicable Applications for SPD recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Ala21~Phe375 (Accession # P35247) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 61699226
• NCBI GeneID: 6441
• NCBI Accession #: NP_003010.4
• NCBI GenBank Nucleotide #: NM_003019.4
• UniProt Accession #: P35247; Q5T0M3; Q6FH08; Q86YK9; Q8TCD8; Q9UCJ2; Q9UCJ3
• Molecular Weight: 37.0kDa
• NCBI Official Full Name: pulmonary surfactant-associated protein D
• NCBI Official Synonym Full Names: surfactant protein D
• NCBI Official Symbol: SFTPD
• NCBI Official Synonym Symbols: SP-D; PSP-D; SFTP4; COLEC7
• NCBI Protein Information: pulmonary surfactant-associated protein D; collectin 7; collectin-7; lung surfactant protein D; pulmonary surfactant apoprotein; surfactant, pulmonary-associated protein D; surfactant-associated protein, pulmonary 4
• UniProt Protein Name: Pulmonary surfactant-associated protein D
• UniProt Gene Name: SFTPD
• UniProt Synonym Gene Names: COLEC7; PSPD; SFTP4; PSP-D; SP-D
• UniProt Entry Name: SFTPD_HUMAN

NCBI Description

The protein encoded by this gene is part of the innate immune response, protecting the lungs against inhaled microorganisms and chemicals. The encoded protein may also be involved in surfactant metabolism. [provided by RefSeq, Jul 2015]

Uniprot Description

SFTPD: Contributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. Belongs to the SFTPD family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 10q22.2-q23.1

Cellular Component: extracellular space; proteinaceous extracellular matrix; collagen; lysosome; endocytic vesicle; extracellular region

Molecular Function: protein binding; carbohydrate binding

Biological Process: receptor-mediated endocytosis; negative regulation of T cell proliferation; positive regulation of phagocytosis; negative regulation of interleukin-2 biosynthetic process; defense response to bacterium; innate immune response; respiratory gaseous exchange; alveolus development; macrophage chemotaxis; surfactant homeostasis; regulation of cytokine production