Histidine Rich Glycoprotein (HRG) Recombinant Protein | HRG recombinant protein

Recombinant Histidine Rich Glycoprotein (HRG)

Cat. Num. AAA2010965

• Gene Names: HRG; HPRG; HRGP; THPH11
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Histidine Rich Glycoprotein (HRG); Recombinant Histidine Rich Glycoprotein (HRG); HRG recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-AVS PTDCSAVEPE AEKALDLINK RRRDGYLFQL LRIADAHLDR VENTTVYYLV LDVQESDCSV LSRKYWNDCE PPDSRRPSEI VIGQCKVIAT RHSHESQDLR VIDFNCTTSS VSSALANTKD SPVLIDFFED TERYRKQANK ALEKYKEEND DFASFRVDRI ERVARVRGGE GTGYFVDFSV RNCPRHHFPR HPNVFGFCRA DLFYDVEALD LESPKNLVIN CEVFDPQEHE NING
• Sequence Length: 525

• Applicable Applications for HRG recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Ala18~Gly254 (Accession # P04196) with two N-terminal Tags, His-tag and GST-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4504489
• NCBI GeneID: 3273
• NCBI Accession #: NP_000403.1
• NCBI GenBank Nucleotide #: NM_000412.3
• UniProt Accession #: P04196; B9EK35; D3DNU7
• Molecular Weight: 59.2kDa
• NCBI Official Full Name: histidine-rich glycoprotein
• NCBI Official Synonym Full Names: histidine-rich glycoprotein
• NCBI Official Symbol: HRG
• NCBI Official Synonym Symbols: HPRG; HRGP; THPH11
• NCBI Protein Information: histidine-rich glycoprotein; histidine-proline-rich glycoprotein
• UniProt Protein Name: Histidine-rich glycoprotein
• UniProt Gene Name: HRG
• UniProt Synonym Gene Names: HPRG
• UniProt Entry Name: HRG_HUMAN

NCBI Description

This histidine-rich glycoprotein contains two cystatin-like domains and is located in plasma and platelets. The physiological function has not been determined but it is known that the protein binds heme, dyes and divalent metal ions. The encoded protein also has a peptide that displays antimicrobial activity against C. albicans, E. coli, S. aureus, P. aeruginosa, and E. faecalis. It can inhibit rosette formation and interacts with heparin, thrombospondin and plasminogen. Two of the protein's effects, the inhibition of fibrinolysis and the reduction of inhibition of coagulation, indicate a potential prothrombotic effect. Mutations in this gene lead to thrombophilia due to abnormal histidine-rich glycoprotein levels. [provided by RefSeq, Nov 2014]

Uniprot Description

HRG: Plasma glycoprotein that binds a number of ligands such as heme, heparin, heparan sulfate, thrombospondin, plasminogen, and divalent metal ions. Binds heparin and heparin/glycosaminoglycans in a zinc-dependent manner. Binds heparan sulfate on the surface of liver, lung, kidney and heart endothelial cells. Binds to N-sulfated polysaccharide chains on the surface of liver endothelial cells. Inhibits rosette formation. Acts as an adapter protein and is implicated in regulating many processes such as immune complex and pathogen clearance, cell chemotaxis, cell adhesion, angiogenesis, coagulation and fibrinolysis. Mediates clearance of necrotic cells through enhancing the phagocytosis of necrotic cells in an heparan sulfate-dependent pathway. This process can be regulated by the presence of certain HRG ligands such as heparin and zinc ions. Binds to IgG subclasses of immunoglobins containing kappa and lambda light chains with different affinities regulating their clearance and inhibiting the formation of insoluble immune complexes. Tethers plasminogen to the cell surface. Binds T-cells and alters the cell morphology. Modulates angiogenesis by blocking the CD6-mediated antiangiongenic effect of thrombospondins, THBS1 and THBS2. Acts as a regulator of the vascular endothelial growth factor (VEGF) signaling pathway; inhibits endothelial cell motility by reducing VEGF-induced complex formation between PXN/paxillin and ILK/integrin-linked protein kinase and by promoting inhibition of VEGF-induced tyrosine phosphorylation of focal adhesion kinases and alpha-actinins in endothelial cells. Also plays a role in the regulation of tumor angiogenesis and tumor immune surveillance. Normalizes tumor vessels and promotes antitumor immunity by polarizing tumor-associated macrophages, leading to decreased tumor growth and metastasis. Defects in HRG are the cause of thrombophilia due to histidine-rich glycoprotein deficiency (THPH11). A hemostatic disorder characterized by a tendency to thrombosis.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: plasma membrane; extracellular region

Molecular Function: heparan sulfate proteoglycan binding; heparin binding; serine-type endopeptidase inhibitor activity; protein binding; zinc ion binding; metal ion binding; heme binding; immunoglobulin binding; cysteine protease inhibitor activity; receptor binding

Biological Process: platelet activation; positive regulation of apoptosis; positive regulation of immune response to tumor cell; negative regulation of cell adhesion; chemotaxis; negative regulation of blood vessel endothelial cell migration; response to organic cyclic substance; negative regulation of cell proliferation; fibrinolysis; negative regulation of angiogenesis; negative regulation of fibrinolysis; platelet degranulation; positive regulation of focal adhesion formation; regulation of gene expression; regulation of actin cytoskeleton organization and biogenesis; regulation of blood coagulation; regulation of protein complex assembly; negative regulation of cell growth; angiogenesis; blood coagulation; regulation of peptidyl-tyrosine phosphorylation; negative regulation of cell adhesion mediated by integrin; defense response to fungus

Disease: Thrombophilia Due To Histidine-rich Glycoprotein Deficiency