Interleukin 5 Receptor Alpha (IL5Ra) Recombinant Protein | IL5Ra recombinant protein

Recombinant Interleukin 5 Receptor Alpha (IL5Ra)

Cat. Num. AAA2010734

• Gene Names: IL5RA; IL5R; CD125; CDw125; HSIL5R3
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Interleukin 5 Receptor Alpha (IL5Ra); Recombinant Interleukin 5 Receptor Alpha (IL5Ra); IL5Ra recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-GLAQVLLQW KPNPDQEQRN VNLEYQVKIN APKEDDYETR ITESKCVTIL HKGFSASVRT ILQNDHSLLA SSWASAELHA PPGSPGTSIV NLTCTTNTTE DNYSRLRSYQ VSLHCTWLVG TDAPEDTQYF LYYRYGSWTE EC
• Sequence Length: 420

• Applicable Applications for IL5Ra recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Gly42~Cys182 (Accession # Q01344) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 28559021
• NCBI GeneID: 3568
• NCBI Accession #: NP_000555.2
• NCBI GenBank Nucleotide #: NM_000564.4
• UniProt Accession #: Q01344; Q14633; Q15469; Q6ISX9; B3IU77; B4E2G0
• Molecular Weight: 17.5kDa
• NCBI Official Full Name: interleukin-5 receptor subunit alpha isoform 1
• NCBI Official Synonym Full Names: interleukin 5 receptor, alpha
• NCBI Official Symbol: IL5RA
• NCBI Official Synonym Symbols: IL5R; CD125; CDw125; HSIL5R3
• NCBI Protein Information: interleukin-5 receptor subunit alpha; CD125 antigen; IL-5R subunit alpha; IL-5 receptor subunit alpha; interleukin 5 receptor type 3; interleukin-5 receptor alpha chain
• UniProt Protein Name: Interleukin-5 receptor subunit alpha
• Protein Family: Interleukin-5 receptor
• UniProt Gene Name: IL5RA
• UniProt Synonym Gene Names: IL5R; IL-5 receptor subunit alpha; IL-5R subunit alpha; IL-5R-alpha; IL-5RA
• UniProt Entry Name: IL5RA_HUMAN

NCBI Description

The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Several alternatively spliced transcript variants encoding four distinct isoforms have been reported. [provided by RefSeq, Jul 2011]

Uniprot Description

IL5RA: This is the receptor for interleukin-5. The alpha chain binds to IL5. Belongs to the type I cytokine receptor family. Type 5 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p26-p24

Cellular Component: extracellular space; integral to membrane; plasma membrane

Molecular Function: protein binding; interleukin-5 receptor activity

Biological Process: regulation of interleukin-5 production; cell proliferation; inflammatory response to antigenic stimulus; signal transduction