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SDS-Page

Apolipoprotein A2 (APOA2) Recombinant Protein | APOA2 recombinant protein

Recombinant Apolipoprotein A2 (APOA2)

Gene Names
Apoa2; APOAII; Apo-AII; ApoA-II
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Apolipoprotein A2 (APOA2); Recombinant Apolipoprotein A2 (APOA2); APOA2 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-QAAETDV QTLFSQYLQS LTDYGKDLME KAQPSEIQNQ AKAYFQNAQE RLTPFVQRTG TNLMDFLSRL MSPEEKPAPAAK
Sequence Length
102
Applicable Applications for APOA2 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Rattus norvegicus (Rat)
Expression System
Prokaryotic expression
Residues
Gln24~Lys102 (Accession # P04638) with two N-terminal Tags, His-tag and GST-tag
Subcellular Location
Secreted
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Predicted Isoelectric Point
5.9
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40.9kDa
NCBI Official Full Name
apolipoprotein A-II preproprotein
NCBI Official Synonym Full Names
apolipoprotein A-II
NCBI Official Symbol
Apoa2
NCBI Official Synonym Symbols
APOAII; Apo-AII; ApoA-II
NCBI Protein Information
apolipoprotein A-II; apolipoprotein A2
UniProt Protein Name
Apolipoprotein A-II
Protein Family
UniProt Gene Name
Apoa2
UniProt Synonym Gene Names
Apo-AII; ApoA-II
UniProt Entry Name
APOA2_RAT

NCBI Description

may play a role in lipid metabolism and transport [RGD, Feb 2006]

Uniprot Description

APOA2: May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism. Belongs to the apolipoprotein A2 family.

Protein type: Secreted; Secreted, signal peptide; Lipid-binding; Endoplasmic reticulum

Cellular Component: extracellular space; chylomicron

Molecular Function: lipid transporter activity; protein homodimerization activity; lipase inhibitor activity; protein heterodimerization activity; cholesterol transporter activity; cholesterol binding; phospholipid binding; phosphatidylcholine binding; high-density lipoprotein binding; lipid binding; apolipoprotein receptor binding

Biological Process: positive regulation of catalytic activity; protein folding; negative regulation of cholesterol transport; response to glucocorticoid stimulus; regulation of protein stability; diacylglycerol catabolic process; positive regulation of lipid catabolic process; negative regulation of lipase activity; negative regulation of lipid catabolic process; regulation of cholesterol absorption; phospholipid efflux; response to glucose stimulus; response to drug; cholesterol metabolic process; organ regeneration; cholesterol transport; protein amino acid oxidation; phospholipid catabolic process; positive regulation of interleukin-8 biosynthetic process; lipoprotein metabolic process; cholesterol efflux; lipid transport; negative regulation of cytokine secretion during immune response; cholesterol homeostasis; reverse cholesterol transport; response to estrogen stimulus; peptidyl-methionine modification; phosphatidylcholine biosynthetic process; acute inflammatory response

Research Articles on APOA2

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Product Notes

The APOA2 apoa2 (Catalog #AAA2010195) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Apolipoprotein A2 (APOA2) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the APOA2 apoa2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below. MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF -QAAETDV QTLFSQYLQS LTDYGKDLME KAQPSEIQNQ AKAYFQNAQE RLTPFVQRTG TNLMDFLSRL MSPEEKPAPA AK. It is sometimes possible for the material contained within the vial of "Apolipoprotein A2 (APOA2), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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