Matrix Metalloproteinase 13 (MMP13) Recombinant Protein | MMP13 recombinant protein

Recombinant Matrix Metalloproteinase 13 (MMP13)

Cat. Num. AAA2010156

• Gene Names: Mmp13; Clg; Mmp1; MMP-13
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Matrix Metalloproteinase 13 (MMP13); Recombinant Matrix Metalloproteinase 13 (MMP13); MMP13 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- VDRLR EMQSFFGLEV TGKLDDPTLD IMRKPRCGVP DVGEYNVFPR TLKWSQTNLT YRIVNYTPDM SHSEVEKAFR KAFKVWSDVT PLNFTRIYDG TADIMISFGT KEHGDFYPFD GPSGLLAHAF PPGPNYGGDA HFDDDETWTS SSKGYNLFIV AAHELGHSLG LDHSKDPG
• Sequence Length: 472

• Applicable Applications for MMP13 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Val66~Gly238 (Accession # P33435) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6678896
• NCBI GeneID: 17386
• NCBI Accession #: NP_032633.1
• NCBI GenBank Nucleotide #: NM_008607.2
• UniProt Accession #: P33435
• Molecular Weight: 21.0kDa
• NCBI Official Full Name: collagenase 3 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 13
• NCBI Official Symbol: Mmp13
• NCBI Official Synonym Symbols: Clg; Mmp1; MMP-13
• NCBI Protein Information: collagenase 3; Collagenase-3; collagenase-1; interstitial collagenase; matrix metalloproteinase 13; matrix metalloproteinase-13
• UniProt Protein Name: Collagenase 3
• Protein Family: Collagenase
• UniProt Gene Name: Mmp13
• UniProt Synonym Gene Names: MMP-13
• UniProt Entry Name: MMP13_MOUSE

NCBI Description

This gene encodes a member of the matrix metalloproteinase family that plays a role in wound healing, skeletal development and bone remodeling. The encoded protein is activated by the removal of an N-terminal activation peptide to generate a zinc-dependent endopeptidase enzyme that can cleave various native collagens, including types I - IV, X and XIV. Mice lacking the encoded protein display profound defects in growth plate cartilage as well as a delay in the endochondral bone development. Lack of the encoded protein also impairs the wound healing process due to reduced keratinocyte migration and vascular density at the wound site. This gene is located in a cluster of other matrix metalloproteinase genes on chromosome 9. [provided by RefSeq, Jun 2015]

Uniprot Description

MMP13: Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process. Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO). A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age. Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1). Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia. Belongs to the peptidase M10A family.

Protein type: EC 3.4.24.-; Protease; Secreted, signal peptide; Secreted

Cellular Component: Golgi apparatus; extracellular matrix; intercellular canaliculus; proteinaceous extracellular matrix; extracellular space; fibrillar collagen; lysosome; extracellular region

Molecular Function: collagen binding; peptidase activity; low-density lipoprotein receptor binding; metallopeptidase activity; zinc ion binding; hydrolase activity; metal ion binding; metalloendopeptidase activity; fibronectin binding; calcium ion binding; calcium-dependent protein binding

Biological Process: collagen catabolic process; extracellular matrix disassembly; cellular protein metabolic process; cartilage development; receptor internalization; peptide catabolic process; proteolysis; bone mineralization; endochondral ossification