Endothelin 1 (EDN1) Recombinant Protein | EDN1 recombinant protein

Recombinant Endothelin 1 (EDN1)

Cat. Num. AAA2009978

• Gene Names: EDN1; ET1; PPET1; HDLCQ7
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Endothelin 1 (EDN1); Recombinant Endothelin 1 (EDN1); EDN1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-CSCSSLMD KECVYFCHLD IIWVNTPEHV VPYGLGSPRS
• Sequence Length: 211

• Applicable Applications for EDN1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Cys53~Ser90 (Accession # P05305) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 269995983
• NCBI GeneID: 1906
• NCBI Accession #: NP_001161791.1
• NCBI GenBank Nucleotide #: NM_001168319.1
• UniProt Accession #: P05305; Q96DA1
• Molecular Weight: 5.8kDa
• NCBI Official Full Name: endothelin-1 isoform 2 preproprotein
• NCBI Official Synonym Full Names: endothelin 1
• NCBI Official Symbol: EDN1
• NCBI Official Synonym Symbols: ET1; PPET1; HDLCQ7
• NCBI Protein Information: endothelin-1; preproendothelin-1
• UniProt Protein Name: Endothelin-1
• Protein Family: Endothelin
• UniProt Gene Name: EDN1
• UniProt Synonym Gene Names: PPET1; ET-1
• UniProt Entry Name: EDN1_HUMAN

NCBI Description

The protein encoded by this gene is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2009]

Uniprot Description

EDN1: Endothelins are endothelium-derived vasoconstrictor peptides. Belongs to the endothelin/sarafotoxin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6p24.1

Cellular Component: extracellular space; cytoplasm; extracellular region

Molecular Function: protein binding; hormone activity; endothelin B receptor binding; cytokine activity; endothelin A receptor binding

Biological Process: response to nicotine; positive regulation of JNK activity; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of nitric oxide biosynthetic process; regulation of systemic arterial blood pressure by endothelin; heart development; response to lipopolysaccharide; middle ear morphogenesis; sensory perception of pain; prostaglandin biosynthetic process; positive regulation of MAP kinase activity; elevation of cytosolic calcium ion concentration; negative regulation of cAMP biosynthetic process; cell surface receptor linked signal transduction; cell-cell signaling; negative regulation of nitric-oxide synthase biosynthetic process; protein kinase C activation; cell growth; neutrophil chemotaxis; rhythmic excitation; positive regulation of mitosis; negative regulation of blood coagulation; positive regulation of heart rate; response to testosterone stimulus; respiratory gaseous exchange; peptide hormone secretion; response to amino acid stimulus; leukocyte activation; patterning of blood vessels; membrane depolarization; protein kinase C deactivation; regulation of vasoconstriction; positive regulation of transcription from RNA polymerase II promoter; response to activity; superoxide release; positive regulation of odontogenesis; epithelial fluid transport; phosphoinositide 3-kinase cascade; G-protein signaling, phospholipase D activating pathway; positive regulation of cell size; neural crest cell development; negative regulation of hormone secretion; positive regulation of smooth muscle cell proliferation; negative regulation of cellular protein metabolic process; glucose transport; negative regulation of transcription from RNA polymerase II promoter; vein smooth muscle contraction; histamine secretion; nitric oxide transport; positive regulation of cell proliferation; regulation of pH; positive regulation of smooth muscle contraction; artery smooth muscle contraction; vasoconstriction; inositol phosphate-mediated signaling; in utero embryonic development; positive regulation of hormone secretion; calcium-mediated signaling; multicellular organismal aging; body fluid secretion; G-protein coupled receptor protein signaling pathway; dorsal/ventral pattern formation; cartilage development; response to ozone; maternal process involved in parturition; positive regulation of prostaglandin secretion; regulation of sensory perception of pain; positive regulation of cell migration

Disease: Question Mark Ears, Isolated; Auriculocondylar Syndrome 3