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Sequence Information

Proteinase 3 (PR3) Recombinant Protein | PR3 recombinant protein

Recombinant Proteinase 3 (PR3)

Gene Names
PRTN3; MBN; MBT; NP4; P29; PR3; ACPA; AGP7; NP-4; PR-3; CANCA; C-ANCA
Applications
SDS-Page, Western Blot
Purity
> 95%
Synonyms
Proteinase 3 (PR3); Recombinant Proteinase 3 (PR3); PR3 recombinant protein
Ordering
For Research Use Only!
Host
E.coli
Purity/Purification
> 95%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Concentration
Original Concentration: 100ug/mL (varies by lot)
Applicable Applications for PR3 recombinant protein
Positive Control (PC), Immunogen, SDS-PAGE (SDS), Western Blot (WB)
Application Notes
(May be suitable for use in other assays to be determined by the end user.)
Organism Species
Homo sapiens (Human)
Source
Prokaryotic expression
Residues
His71~Leu247
Tags
N-terminal His Tag
Subcellular Location
Secreted
Traits
Freeze-dried powder
Predicted isoelectric point
6.6
Usage
Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.

Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration dateunder appropriate storage condition.

Sequence Information

Sequence Information

SDS-Page

SDS-Page

Testing Data

Testing Data

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 20.8kDa
Accurate Molecular Mass: 21/17kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
myeloblastin
NCBI Official Synonym Full Names
proteinase 3
NCBI Official Symbol
PRTN3
NCBI Official Synonym Symbols
MBN; MBT; NP4; P29; PR3; ACPA; AGP7; NP-4; PR-3; CANCA; C-ANCA
NCBI Protein Information
myeloblastin; C-ANCA antigen; wegener autoantigen; leukocyte proteinase 3; neutrophil proteinase 4; azurophil granule protein 7; serine proteinase, neutrophil; Wegener granulomatosis autoantigen
UniProt Protein Name
Myeloblastin
UniProt Gene Name
PRTN3
UniProt Synonym Gene Names
MBN; PR-3; PR3; NP-4
UniProt Entry Name
PRTN3_HUMAN

Uniprot Description

PRTN3: Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters. Belongs to the peptidase S1 family. Elastase subfamily.

Protein type: Protease; Cell cycle regulation; EC 3.4.21.76

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: extracellular matrix; extracellular space; plasma membrane; cytosol

Molecular Function: enzyme binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: negative regulation of phagocytosis; collagen catabolic process; positive regulation of cell proliferation; blood coagulation; proteolysis

Research Articles on PR3

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Product Notes

The PR3 prtn3 (Catalog #AAA2009658) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Proteinase 3 (PR3) can be used in a range of immunoassay formats including, but not limited to, Positive Control (PC), Immunogen, SDS-PAGE (SDS), Western Blot (WB) . (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the PR3 prtn3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Proteinase 3 (PR3), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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