mug cdna clone
mug
Gene Names
mug; ECK3058; ygjF
Synonyms
mug; dug; ECK3058; JW3040; ygjF; mug cdna clone
Form/Format
Lyophilized
Sequence
Nucleotide Sequence: ATGGTTGAGGATATTTTGGCTCCAGGGTTACGGGTCGTGTTTTGCGGTATCAACCCTGGGCTTTCATCCGCCGGGACTGGTTTTCCCTTTGCTCATCCGGCAAATCGCTTCTGGAAGGTGATATATCAGGCCGGGTTTACCGACCGTCAGTTGAAGCCGCAGGAGGCACAGCATCTGCTGGATTATCGTTGTGGCGTCACCAAACTGGTAGACCGTCCAACGGTGCAAGCCAATGAAGTTTCAAAGCAGGAGCTACACGCAGGCGGGCGTAAGCTGATTGAAAAAATTGAAGATTATCAGCCGCAGGCGTTGGCGATTCTGGGCAAACAAGCATATGAACAGGGATTCAGCCAGCGCGGTGCACAGTGGGGGAAACAAACGCTCACCATTGGTTCGACGCAGATTTGGGTGCTGCCAAATCCCAGCGGTTTAAGTCGCGTTTCACTGGAGAAACTGGTTGAAGCGTATCGCGAGCTGGACCAGGCGCTGGTAGTGCGTGGGCGATAA
Translation Sequence: MVEDILAPGL RVVFCGINPG LSSAGTGFPF AHPANRFWKV IYQAGFTDRQ LKPQEAQHLL DYRCGVTKLV DRPTVQANEV SKQELHAGGR KLIEKIEDYQ PQALAILGKQ AYEQGFSQRG AQWGKQTLTI GSTQIWVLPN PSGLSRVSLE KLVEAYRELD QALVVRGR
Translation Sequence: MVEDILAPGL RVVFCGINPG LSSAGTGFPF AHPANRFWKV IYQAGFTDRQ LKPQEAQHLL DYRCGVTKLV DRPTVQANEV SKQELHAGGR KLIEKIEDYQ PQALAILGKQ AYEQGFSQRG AQWGKQTLTI GSTQIWVLPN PSGLSRVSLE KLVEAYRELD QALVVRGR
Sequence Length
168
Species
E Coli
cDNA Size
507bp
Vector Description
This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
Vector
(puc19-derived cloning vector)
Preparation Before Usage
1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid.
Preparation and Storage
Store the plasmid at -20 degree C.
Related Product Information for mug cdna clone
G/u mismatch-specific DNA glycosylase, xanthine DNA glycosylase, also known as mug, belongs to the TDG/mug DNA glycosylase family. It has been proposed that the Mug protein excises 3, N4-ethenocytosine and removes the uracil base from mismatches in the order of u:G>u:A, although the biological role remains unclear.
Product Categories/Family for mug cdna clone
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
stationary phase mismatch/uracil DNA glycosylase
NCBI Official Symbol
mug
NCBI Official Synonym Symbols
ECK3058; ygjF
NCBI Protein Information
stationary phase mismatch/uracil DNA glycosylase
UniProt Protein Name
G/U mismatch-specific DNA glycosylase
Protein Family
UniProt Gene Name
mug
UniProt Synonym Gene Names
ygjF; MUG
UniProt Entry Name
MUG_ECOLI
NCBI Description
Endonuclease IV stimulates Mug activity in vitro by promoting disassociation from the DNA and enzyme turnover. [More information is available at EcoGene: EG12717]. Mug is a glycosylase that acts in DNA repair . [More information is available at EcoCyc: EG12717].
Uniprot Description
Excises ethenocytosine and uracil, which can arise by alkylation or deamination of cytosine, respectively, from the corresponding mispairs with guanine in ds-DNA. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired base. The complementary strand guanine functions in substrate recognition. Required for DNA damage lesion repair in stationary-phase cells.
Research Articles on mug
Similar Products
Product Notes
The mug mug (Catalog #AAA200953) is a cDNA Clone and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Nucleotide Sequence: ATGGTTGAGG ATATTTTGGC TCCAGGGTTA CGGGTCGTGT TTTGCGGTAT CAACCCTGGG CTTTCATCCG CCGGGACTGG TTTTCCCTTT GCTCATCCGG CAAATCGCTT CTGGAAGGTG ATATATCAGG CCGGGTTTAC CGACCGTCAG TTGAAGCCGC AGGAGGCACA GCATCTGCTG GATTATCGTT GTGGCGTCAC CAAACTGGTA GACCGTCCAA CGGTGCAAGC CAATGAAGTT TCAAAGCAGG AGCTACACGC AGGCGGGCGT AAGCTGATTG AAAAAATTGA AGATTATCAG CCGCAGGCGT TGGCGATTCT GGGCAAACAA GCATATGAAC AGGGATTCAG CCAGCGCGGT GCACAGTGGG GGAAACAAAC GCTCACCATT GGTTCGACGC AGATTTGGGT GCTGCCAAAT CCCAGCGGTT TAAGTCGCGT TTCACTGGAG AAACTGGTTG AAGCGTATCG CGAGCTGGAC CAGGCGCTGG TAGTGCGTGG GCGATAATrans lation Sequence: MVEDILAPGL RVVFCGINPG LSSAGTGFPF AHPANRFWKV IYQAGFTDRQ LKPQEAQHLL DYRCGVTKLV DRPTVQANEV SKQELHAGGR KLIEKIEDYQ PQALAILGKQ AYEQGFSQRG AQWGKQTLTI GSTQIWVLPN PSGLSRVSLE KLVEAYRELD QALVVRGR. It is sometimes possible for the material contained within the vial of "mug, cDNA Clone" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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