Glutamate Cysteine Ligase, Catalytic (GCLC) Recombinant Protein | GCLC recombinant protein

Recombinant Glutamate Cysteine Ligase, Catalytic (GCLC)

Cat. Num. AAA2009208

• Gene Names: Gclc; Glclc
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Glutamate Cysteine Ligase; Catalytic (GCLC); Recombinant Glutamate Cysteine Ligase; GCLC recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG SEF-DFENSA YVVFVVLLTR VILSYKLDFL IPLSKVDENM KVAQERDAVL QGMFYFRKDI CKGGNAVVDG CSKAQTSSEP SAEEYTLMSI DTIINGKEGV FPGLIPILNS YLENMEVDVD TRCSILNYLK LIKKRASGEL MTVARWMREF IANHPDYKQD SVITDEINYS LILKCNQIAN ELCECPELLG SGFRKAKYSG GKSDPS
• Sequence Length: 637

• Applicable Applications for GCLC recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Asp435~Ser636 (Accession # P19468) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 25742748
• NCBI GeneID: 25283
• NCBI Accession #: NP_036947.1
• NCBI GenBank Nucleotide #: NM_012815.2
• UniProt Accession #: P19468
• Molecular Weight: 24.2kDa
• NCBI Official Full Name: glutamate--cysteine ligase catalytic subunit
• NCBI Official Synonym Full Names: glutamate-cysteine ligase, catalytic subunit
• NCBI Official Symbol: Gclc
• NCBI Official Synonym Symbols: Glclc
• NCBI Protein Information: glutamate--cysteine ligase catalytic subunit; gamma-ECS; GCS heavy chain; gamma-glutamylcysteine synthetase; Glutamylcysteine gamma synthetase light chain
• UniProt Protein Name: Glutamate--cysteine ligase catalytic subunit
• Protein Family: Glutamate--cysteine ligase
• UniProt Gene Name: Gclc
• UniProt Synonym Gene Names: Glclc
• UniProt Entry Name: GSH1_RAT

NCBI Description

catalyzes the first step in the synthesis of glutathione [RGD, Feb 2006]

Uniprot Description

GCLC: Defects in GCLC are the cause of hemolytic anemia (HAGGSD). Belongs to the glutamate--cysteine ligase type 3 family.

Protein type: Ligase; Other Amino Acids Metabolism - glutathione; EC 6.3.2.2

Cellular Component: glutamate-cysteine ligase complex; cytosol

Molecular Function: glutamate binding; protein heterodimerization activity; glutamate-cysteine ligase activity; magnesium ion binding; coenzyme binding; ADP binding; ATP binding

Biological Process: glutamate metabolic process; response to arsenic; L-ascorbic acid metabolic process; response to hormone stimulus; response to nitrosative stress; response to xenobiotic stimulus; cysteine metabolic process; apoptotic mitochondrial changes; glutathione metabolic process; cell redox homeostasis; response to heat; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; glutathione biosynthetic process; negative regulation of protein ubiquitination; negative regulation of neuron apoptosis; response to oxidative stress; regulation of blood vessel size; negative regulation of transcription, DNA-dependent; regulation of mitochondrial depolarization; negative regulation of apoptosis