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Fatty Acid Transport Protein 5 (FATP5) Recombinant Protein | FATP5 recombinant protein

Recombinant Fatty Acid Transport Protein 5 (FATP5)

Gene Names
Slc27a5; FATP5; FACVL3; VLCSH2; Vlacsr; VLCS-H2
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Fatty Acid Transport Protein 5 (FATP5); Recombinant Fatty Acid Transport Protein 5 (FATP5); FATP5 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF- ETAEPLRDKQ GFCIPVEPGK PGLLLTKVRK NQPFLGYRGS QAESNRKLVA NVRRVGDLYF NTGDVLTLDQ EGFFYFQDRL GDTFRWKGEN VSTGEVECVL SSLDFLEEVN VYGVPVPGCE GKVGMAAVKL APGKTFDGQK LYQHVRSWLP AYATPHFIRI QDSLEITNTY KLVKSRLVRE GFDVGIIADP LYILDNKAQT FRSLMPDVYQ AVCEGTWNL
Sequence Length
689
Applicable Applications for FATP5 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Mus musculus (Mouse)
Expression System
Prokaryotic expression
Residues
Glu471~Leu689 (Accession # Q4LDG0) with N-terminal His-Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
26.1kDa
NCBI Official Full Name
bile acyl-CoA synthetase
NCBI Official Synonym Full Names
solute carrier family 27 (fatty acid transporter), member 5
NCBI Official Symbol
Slc27a5
NCBI Official Synonym Symbols
FATP5; FACVL3; VLCSH2; Vlacsr; VLCS-H2
NCBI Protein Information
bile acyl-CoA synthetase; BAL; BACS; FATP-5; BA-CoA ligase; VLACS-related; cholate--CoA ligase; bile acid-CoA ligase; fatty acid transport protein 5; solute carrier family 27 member 5; very long-chain acyl-CoA synthetase-related protein; very long-chain acyl-Coenzyme A dehydrogenase synthase related
UniProt Protein Name
Bile acyl-CoA synthetase
UniProt Gene Name
Slc27a5
UniProt Synonym Gene Names
Acsb; Acsvl6; Fatp5; Vlacsr; BACS; BA-CoA ligase; BAL; FATP-5; VLACS-related; VLACSR
UniProt Entry Name
S27A5_MOUSE

Uniprot Description

SLC27A5: Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation. In vitro, also activates 3-alpha,7- alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol. Belongs to the ATP-dependent AMP-binding enzyme family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Lipid Metabolism - primary bile acid biosynthesis; EC 6.2.1.7; Endoplasmic reticulum; Ligase

Cellular Component: protein complex; intracellular membrane-bound organelle; membrane; endoplasmic reticulum; integral to membrane; basal plasma membrane; integral to endoplasmic reticulum membrane

Molecular Function: cholate-CoA ligase activity; fatty acid transporter activity; nucleotide binding; protein complex binding; very-long-chain-fatty-acid-CoA ligase activity; catalytic activity; ATP binding; ligase activity; long-chain-fatty-acid-CoA ligase activity

Biological Process: bile acid biosynthetic process; bile acid metabolic process; very-long-chain fatty acid metabolic process; metabolic process; ketone body biosynthetic process; lipid metabolic process; long-chain fatty acid metabolic process; triacylglycerol mobilization; fatty acid metabolic process; plasma membrane long-chain fatty acid transport; fatty acid transport

Research Articles on FATP5

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Product Notes

The FATP5 slc27a5 (Catalog #AAA2009130) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Fatty Acid Transport Protein 5 (FATP5) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the FATP5 slc27a5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSG SEF- ETAEPLRDKQ GFCIPVEPGK PGLLLTKVRK NQPFLGYRGS QAESNRKLVA NVRRVGDLYF NTGDVLTLDQ EGFFYFQDRL GDTFRWKGEN VSTGEVECVL SSLDFLEEVN VYGVPVPGCE GKVGMAAVKL APGKTFDGQK LYQHVRSWLP AYATPHFIRI QDSLEITNTY KLVKSRLVRE GFDVGIIADP LYILDNKAQT FRSLMPDVYQ AVCEGTWNL. It is sometimes possible for the material contained within the vial of "Fatty Acid Transport Protein 5 (FATP5), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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