PIAS4 cdna clone

PIAS4

Cat. Num. AAA200898

• Gene Names: PIAS4; PIASY; Piasg; ZMIZ6; PIAS-gamma
• Synonyms: PIAS4; PIAS-gamma; Piasg; PIASY; ZMIZ6; PIAS4 cdna clone

• Form/Format: Lyophilized
• Sequence: Nucleotide Sequence: ATGGCGGCGGAGCTGGTGGAGGCCAAAAACATGGTGATGAGTTTTCGAGTCTCCGACCTTCAGATGCTCCTGGGTTTCGTGGGCCGGAGTAAGAGTGGACTGAAGCACGAGCTCGTCACCAGGGCCCTCCAGCTGGTGCAGTTTGACTGTAGCCCTGAGCTGTTCAAGAAGATCAAGGAGCTGTACGAGACCCGCTACGCCAAGAAGAACTCGGAGCCTGCCCCACAGCCGCACCGGCCCCTGGACCCCCTGACCATGCACTCCACCTACGACCGGGCCGGCGCTGTGCCCAGGACTCCGCTGGCAGGCCCCAATATTGACTACCCCGTGCTCTACGGAAAGTACTTAAACGGACTGGGACGGTTGCCCGCCAAGACCCTCAAGCCAGAAGTCCGCCTGGTGAAGCTGCCGTTCTTTAATATGCTGGATGAGCTGCTGAAGCCCACCGAATTAGTCCCACAGAACAACGAGAAGCTTCAGGAGAGCCCGTGCATCTTCGCATTGACGCCAAGACAGGTGGAGTTGATCCGGAACTCCAGGGAACTGCAGCCCGGAGTTAAAGCCGTGCAGGTCGTCCTGAGAATCTGTTACTCAGACACCAGCTGCCCTCAGGAGGACCAGTACCCGCCCAACATCGCTGTGAAGGTCAACCACAGCTACTGCTCCGTCCCGGGCTACTACCCCTCCAATAAGCCCGGGGTGGAGCCCAAGAGGCCGTGCCGCCCCATCAACCTCACTCACCTCATGTACCTGTCCTCGGCCACCAACCGCATCACTGTCACCTGGGGGAACTACGGCAAGAGCTACTCGGTGGCCCTGTACCTGGTGCGGCAGCTGACCTCATCGGAGCTGCTGCAGAGGCTGAAGACCATTGGGGTAAAGCACCCGGAGCTGTGCAAGGCACTGGTCAAGGAGAAGCTGCGCCTTGATCCTGACAGCGAGATCGCCACCACCGGTGTGCGGGTGTCCCTCATCTGTCCGCTGGTGAAGATGCGGCTCTCCGTGCCCTGCCGGGCAGAGACCTGCGCCCACCTGCAGTGCTTCGACGCCGTCTTCTACCTGCAGATGAACGAGAAGAAGCCCACCTGGATGTGCCCCGTGTGCGACAAGCCAGCCCCCTACGACCAGCTCATCATCGACGGGCTCCTCTCGAAGATCCTGAGCGAGTGTGAGGACGCCGACGAGATCGAGTACCTGGTGGACGGCTCGTGGTGCCCGATCCGCGCCGAAAAGGAGCGCAGCTGCAGCCCGCAGGGCGCCATCCTCGTGCTGGGCCCCTCGGACGCCAATGGGCTCCTGCCCGCCCCCAGCGTCAACGGGAGCGGTGCCCTGGGCAGCACGGGTGGCGGCGGCCCGGTGGGCAGCATGGAGAATGGGAAGCCGGGCGCCGATGTGGTGGACCTCACGCTGGACAGCTCATCGTCCTCGGAGGATGAGGAGGAGGAGGAAGAGGAGGAGGAAGACGAGGACGAAGAGGGGCCCCGGCCCAAGCGCCGCTGCCCCTTCCAGAAGGGCCTGGTGCCGGCCTGCTGA; ; Translation Sequence: MAAELVEAKN MVMSFRVSDL QMLLGFVGRS KSGLKHELVT RALQLVQFDC SPELFKKIKELYETRYAKKN SEPAPQPHRP LDPLTMHSTY DRAGAVPRTP LAGPNIDYPV LYGKYLNGLGRLPAKTLKPE VRLVKLPFFN MLDELLKPTE LVPQNNEKLQ ESPCIFALTP RQVELIRNSRELQPGVKAVQ VVLRICYSDT SCPQEDQYPP NIAVKVNHSY CSVPGYYPSN KPGVEPKRPCRPINLTHLMY LSSATNRITV TWGNYGKSYS VALYLVRQLT SSELLQRLKT IGVKHPELCKALVKEKLRLD PDSEIATTGV RVSLICPLVK MRLSVPCRAE TCAHLQCFDA VFYLQMNEKKPTWMCPVCDK PAPYDQLIID GLLSKILSEC EDADEIEYLV DGSWCPIRAE KERSCSPQGAILVLGPSDAN GLLPAPSVNG SGALGSTGGG GPVGSMENGK PGADVVDLTL DSSSSSEDEEEEEEEEEDED EEGPRPKRRC PFQKGLVPAC
• Sequence Length: 510

• Species: Human
• Chromosome Location: 19p13.3
• OMIM Reference Number: 605989
• cDNA Size: 1533bp
• Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
• Vector: (puc19-derived cloning vector)
• Preparation Before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid.
• Preparation and Storage: Store the plasmid at -20 degree C.

Related Product Information for PIAS4 cdna clone

PIAS4 belongs to the PIAS family and highly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes.

Product Categories/Family for PIAS4 cdna clone

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 24850133
• NCBI GeneID: 51588
• NCBI Accession #: NP_056981.2
• NCBI GenBank Nucleotide #: NM_015897.3
• UniProt Accession #: Q8N2W9
• NCBI Official Full Name: E3 SUMO-protein ligase PIAS4
• NCBI Official Synonym Full Names: protein inhibitor of activated STAT 4
• NCBI Official Symbol: PIAS4
• NCBI Official Synonym Symbols: PIASY; Piasg; ZMIZ6; PIAS-gamma
• NCBI Protein Information: E3 SUMO-protein ligase PIAS4
• UniProt Protein Name: E3 SUMO-protein ligase PIAS4
• Protein Family: E3 SUMO-protein ligase
• UniProt Gene Name: PIAS4
• UniProt Synonym Gene Names: PIASG; PIAS-gamma
• UniProt Entry Name: PIAS4_HUMAN

Uniprot Description

PIAS4: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Promotes PARK7 sumoylation. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Interacts with AR, AXIN1, GATA2, LEF1, TP53 and STAT1 (IFNG-induced). Binds to AT-rich DNA sequences, known as matrix or scaffold attachment regions (MARs/SARs). Interacts with TICAM1. Interacts with KLF8; the interaction results in SUMO ligation and repression of KLF8 transcriptional activity and of its cell cycle progression into G(1) phase. Highly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes. Belongs to the PIAS family.

Protein type: EC 6.3.2.-; SUMO conjugating system; Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; PML body; nuclear matrix; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; ubiquitin protein ligase binding; SUMO ligase activity; ligase activity

Biological Process: positive regulation of protein sumoylation; Wnt receptor signaling pathway; protein sumoylation; transcription, DNA-dependent; inhibition of NF-kappaB transcription factor; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent