LIN28B cdna clone
LIN28B
Translation Sequence: MAEGGASKGG GEEPGKLPEP AEEESQVLRG TGHCKWFNVR MGFGFISMIN REGSPLDIPV DVFVHQSKLF MEGFRSLKEG EPVEFTFKKS SKGLESIRVT GPGGSPCLGS ERRPKGKTLQ KRKPKGDRCY NCGGLDHHAK ECSLPPQPKK CHYCQSIMHM VANCPHKNVA QPPASSQGRQ EAESQPCTST LPREVGGGHG CTSPPFPQEA RAEISERSGR SPQEASSTKS SIAPEEQSKK GPSVQKRKKT
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the lin-28 family, which is characterized by the presence of a cold-shock domain and a pair of CCHC zinc finger domains. This gene is highly expressed in testis, fetal liver, placenta, and in primary human tumors and cancer cell lines. It is negatively regulated by microRNAs that target sites in the 3' UTR, and overexpression of this gene in primary tumors is linked to the repression of let-7 family of microRNAs and derepression of let-7 targets, which facilitates cellular transformation. [provided by RefSeq, Jun 2012]
Uniprot Description
LIN28B: Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre- let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth. Interacts with ZCCHC11/TUT4. Might be negatively regulated by the microRNA let-7b. High expression in testis, fetal liver, placenta and in hepatocellular carcinoma (HCC). Isoform 1 is only detected in moderately and poorly differentiated HCC tissues and placenta. Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues. Belongs to the lin-28 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 6q21
Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleolus; nucleus
Molecular Function: protein binding; DNA binding; zinc ion binding; RNA binding
Biological Process: regulation of transcription, DNA-dependent; pre-microRNA processing; RNA 3'-end processing
Research Articles on LIN28B
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Product Notes
The LIN28B lin28b (Catalog #AAA200812) is a cDNA Clone and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Nucleotide Sequence: ATGGCCGAAG GCGGGGCTAG CAAAGGTGGT GGAGAAGAGC CCGGGAAGCT GCCGGAGCCG GCAGAGGAGG AATCCCAGGT TTTGCGCGGA ACTGGCCACT GTAAGTGGTT CAATGTGCGC ATGGGATTTG GATTCATCTC CATGATAAAC CGAGAGGGAA GCCCCTTGGA TATTCCAGTC GATGTATTTG TACACCAAAG CAAACTATTC ATGGAAGGAT TTAGAAGCCT AAAAGAAGGA GAACCAGTGG AATTCACATT TAAAAAATCT TCCAAAGGCC TTGAGTCAAT ACGGGTAACA GGACCTGGTG GGAGCCCCTG TTTAGGAAGT GAAAGAAGAC CCAAAGGGAA GACACTACAG AAAAGAAAAC CAAAGGGAGA TAGATGCTAC AACTGTGGTG GCCTTGATCA TCATGCTAAG GAATGTAGTC TACCTCCTCA GCCAAAGAAG TGCCATTACT GTCAGAGCAT CATGCACATG GTGGCAAACT GCCCACATAA AAATGTTGCA CAGCCACCCG CGAGTTCTCA GGGAAGACAG GAAGCAGAAT CCCAGCCATG CACTTCAACT CTCCCTCGAG AAGTGGGAGG CGGGCATGGC TGTACATCAC CACCGTTTCC TCAGGAGGCT AGGGCAGAGA TCTCAGAACG GTCAGGCAGG TCACCTCAAG AAGCTTCCTC CACGAAGTCA TCTATAGCAC CAGAAGAGCA AAGCAAAAAG GGGCCTTCAG TTCAAAAAAG GAAAAAGACA TAATranslati on Sequence: MAEGGASKGG GEEPGKLPEP AEEESQVLRG TGHCKWFNVR MGFGFISMIN REGSPLDIPV DVFVHQSKLF MEGFRSLKEG EPVEFTFKKS SKGLESIRVT GPGGSPCLGS ERRPKGKTLQ KRKPKGDRCY NCGGLDHHAK ECSLPPQPKK CHYCQSIMHM VANCPHKNVA QPPASSQGRQ EAESQPCTST LPREVGGGHG CTSPPFPQEA RAEISERSGR SPQEASSTKS SIAPEEQSKK GPSVQKRKKT. It is sometimes possible for the material contained within the vial of "LIN28B, cDNA Clone" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
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