Guinea Pig anti-Rabbit Matrix Metalloproteinase 13 (MMP13) Polyclonal Antibody | anti-MMP13 antibody
Polyclonal Antibody to Matrix Metalloproteinase 13 (MMP13)
Reactivity
Rabbit
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Matrix Metalloproteinase 13 (MMP13); Polyclonal Antibody; Polyclonal Antibody to Matrix Metalloproteinase 13 (MMP13); anti-MMP13 antibody
Host
Guinea Pig
Reactivity
Rabbit
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against MMP13. It has been selected for its ability to recognize MMP13 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGS- E MQSFFGLEVT GKLDDNTLAI MKQPRCGVPD VGEYNVFPRT LKWSQTNLTY RIVNYTPDLT HSEVEKAFKK AFKVWSDVTP LNFTRIHNGT ADIMISFGTK EHGDFYPFDG PSGLLAHAFP PGPNYGGDAH FDDDETWTSS SKGYNLFLVA AHEFGHSLGL DHSKDPGALM FPIYT
MGHHHHHHSGS- E MQSFFGLEVT GKLDDNTLAI MKQPRCGVPD VGEYNVFPRT LKWSQTNLTY RIVNYTPDLT HSEVEKAFKK AFKVWSDVTP LNFTRIHNGT ADIMISFGTK EHGDFYPFDG PSGLLAHAFP PGPNYGGDAH FDDDETWTSS SKGYNLFLVA AHEFGHSLGL DHSKDPGALM FPIYT
Sequence Length
471
Applicable Applications for anti-MMP13 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant MMP13 (Glu70~Thr245) expressed in E.coli.
Cross Reactivity
Rabbit
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Rabbit Serum; Primary Ab: 5ug/ml Cavia Anti-Rabbit MMP13 Antibody Second Ab: 0.2ug/mL HRP-Linked Rabbit Anti-Cavia IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
53,693 Da
NCBI Official Full Name
collagenase 3
NCBI Official Symbol
MMP13
NCBI Protein Information
collagenase 3
UniProt Protein Name
Collagenase 3
Protein Family
UniProt Gene Name
MMP13
UniProt Synonym Gene Names
MMP-13
Uniprot Description
Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CTGF. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion ().
