Western Blot (WB)
(Figure. Western Blot; Sample: Recombinant NEFL, Human.)
Rabbit Neurofilament, Light Polypeptide (NEFL) Polyclonal Antibody | anti-NEFL antibody
Polyclonal Antibody to Neurofilament, Light Polypeptide (NEFL)
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Figure. Western Blot; Sample: Recombinant NEFL, Human.)
Western Blot (WB)
(Figure. Western Blot; Sample: Recombinant NEFL, Human.)
Western Blot (WB)
(Western Blot; Sample: Rat Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Human NEFL AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: MBS2086047))
Western Blot (WB)
(Western Blot; Sample: Rat Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Human NEFL AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: MBS2086047))
Western Blot (WB)
(Western Blot; Sample: Mouse Cerebrum lysate;Primary Ab: 1ug/ml Rabbit Anti-Human NEFL AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: MBS2086047))
Western Blot (WB)
(Western Blot; Sample: Mouse Cerebrum lysate;Primary Ab: 1ug/ml Rabbit Anti-Human NEFL AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]
Uniprot Description
NFL: one of the three (L, M, and H) intermediate filament proteins that form neurofilaments. Neurofilaments are involved in the maintenance of neuronal caliber. NF-L is the most abundant of the three neurofilament proteins. Defects cause Charcot-Marie-Tooth disease type 1F (CMT1F).
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 8p21.2
Cellular Component: axon; axon cytoplasm; cytoplasm; cytosol; growth cone; myelin sheath; neurofilament; TSC1-TSC2 complex
Molecular Function: identical protein binding; phospholipase binding; protein binding; protein binding, bridging; protein C-terminus binding; protein domain specific binding; protein heterodimerization activity; Ras guanyl-nucleotide exchange factor activity; structural constituent of cytoskeleton
Biological Process: anterograde axon cargo transport; axon regeneration in the peripheral nervous system; axonal transport of mitochondrion; cerebral cortex development; hippocampus development; intermediate filament organization; intermediate filament polymerization and/or depolymerization; locomotion; MAPK cascade; microtubule cytoskeleton organization; negative regulation of neuron apoptosis; neurofilament bundle assembly; neuromuscular process controlling balance; neuron projection morphogenesis; positive regulation of axonogenesis; protein polymerization; regulation of axon diameter; regulation of molecular function; response to acrylamide; response to corticosterone stimulus; response to peptide hormone; response to sodium arsenite; response to toxin; retrograde axon cargo transport; spinal cord development
Disease: Charcot-marie-tooth Disease, Axonal, Type 2e; Charcot-marie-tooth Disease, Demyelinating, Type 1f
Research Articles on NEFL
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Product Notes
The NEFL nefl (Catalog #AAA2006101) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Neurofilament, Light Polypeptide (NEFL) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Neurofilament, Light Polypeptide (NEFL) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the NEFL nefl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neurofilament, Light Polypeptide (NEFL), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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