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Western Blot (WB)
(Western Blot; Sample: Recombinant DEFb2, Mouse.)
Rabbit anti-Mouse Defensin Beta 2 (DEFb2) Antibody | anti-DEFb2 antibody
Polyclonal Antibody to Defensin Beta 2 (DEFb2)
Gene Names
Defb2; BD-2
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Defensin Beta 2 (DEFb2); Polyclonal Antibody to Defensin Beta 2 (DEFb2); anti-DEFb2 antibody
Host
Rabbit
Reactivity
Mouse
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
0.55mg/ml (varies by lot)
Applicable Applications for anti-DEFb2 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Source
Polyclonal antibody preparation
Cross Reactivity
Mouse
Traits
Liquid
Immunogen
Recombinant DEFb2 (Ala21~Lys71) expressed in E.coli ( MBS2010818 ).
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot; Sample: Recombinant DEFb2, Mouse.)
Western Blot (WB)
(Western Blot; Sample: Recombinant DEFb2, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Mouse Spleen Tissue;Primary Ab: 10ug/ml Rabbit Anti-Mouse DEFb2 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Mouse Spleen Tissue;Primary Ab: 10ug/ml Rabbit Anti-Mouse DEFb2 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue; Primary Ab: 10ug/ml Rabbit Anti-Mouse DEFb2 Ant)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue; Primary Ab: 10ug/ml Rabbit Anti-Mouse DEFb2 Ant)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue; Primary Ab: 10ug/ml Rabbit Anti-Mouse DEFb2 Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue; Primary Ab: 10ug/ml Rabbit Anti-Mouse DEFb2 Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
beta-defensin 2
NCBI Official Synonym Full Names
defensin beta 2
NCBI Official Symbol
Defb2
NCBI Official Synonym Symbols
BD-2
NCBI Protein Information
beta-defensin 2
UniProt Protein Name
Beta-defensin 2
UniProt Gene Name
Defb2
UniProt Synonym Gene Names
BD-2; mBD-2
Uniprot Description
Has bactericidal activity.
Research Articles on DEFb2
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Product Notes
The DEFb2 defb2 (Catalog #AAA2005685) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Defensin Beta 2 (DEFb2) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Defensin Beta 2 (DEFb2) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB), Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the DEFb2 defb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Defensin Beta 2 (DEFb2), Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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