Rabbit anti-Rat Integrin Alpha M (ITGaM) Polyclonal Antibody | anti-ITGaM antibody

Polyclonal Antibody to Integrin Alpha M (ITGaM)

Cat. Num. AAA2005608

• Reactivity: Rat
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Integrin Alpha M (ITGaM); Polyclonal Antibody; Polyclonal Antibody to Integrin Alpha M (ITGaM); anti-ITGaM antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against ITGaM. It has been selected for its ability to recognize ITGaM in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.; MGHHHHHHSGSEF- ARGFGQSVIQ LGETRVVVAA PQEVKAVNQT GALYQCDYST NRCDPIPLQV PPEAVNMSLG LSLAATTVPP QLLACGPTVH QNCKENTYVN GLCYLFGSNL LRKPQQFPEA LRGCPQQESD IAFLIDGSGS
• Sequence Length: 1153

• Applicable Applications for anti-ITGaM antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant ITGaM (Ala31~Ser160) expressed in E.coli.
• Cross Reactivity: Rat
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2057906
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Human Jurkat cell lysate; Primary Ab: 1.5ug/ml Rabbit Anti-Rat ITGaM Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P. Samples: Rat Tissue))

NCBI and Uniprot Product Information

• UniProt Accession #: G3V8L7
• Molecular Weight: 127,192 Da
• UniProt Protein Name: Integrin alpha M
• Protein Family: Integrin
• UniProt Gene Name: Itgam

NCBI Description

This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

ITGAM: a single-pass type I membrane protein of the integrin alpha chain family. Implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. Also known as CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. A receptor for fibrinogen, factor X and ICAM1. Heterodimer of an alpha and a beta subunit. Alpha-M associates with beta-2. Predominantly expressed in monocytes and granulocytes.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cell surface; plasma membrane; integrin complex; nucleus; external side of plasma membrane

Molecular Function: heparin binding; heparan sulfate proteoglycan binding; opsonin binding; protein binding; protein heterodimerization activity; metal ion binding; glycoprotein binding

Biological Process: integrin-mediated signaling pathway; neutrophil chemotaxis; extracellular matrix organization and biogenesis; microglia development; activated T cell proliferation; leukocyte migration during inflammatory response; cellular extravasation; toll-like receptor signaling pathway; innate immune response; blood coagulation; cell adhesion; toll-like receptor 4 signaling pathway; leukocyte migration

Disease: Systemic Lupus Erythematosus, Susceptibility To, 6