Western Blot (WB)
(Figure. Western Blot; Sample: Recombinant ESAM, Mouse.)
Rabbit anti-Mouse Endothelial Cell Adhesion Molecule (ESAM) Antibody | anti-ESAM antibody
Polyclonal Antibody to Endothelial Cell Adhesion Molecule (ESAM)
Gene Names
Esam; Esam1; W117m; 2310008D05Rik
Reactivity
Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Endothelial Cell Adhesion Molecule (ESAM); Polyclonal Antibody to Endothelial Cell Adhesion Molecule (ESAM); anti-ESAM antibody
Host
Rabbit
Reactivity
Mouse
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-ESAM antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
Western blotting: 0.5-2 ug/mL
Immunohistochemistry: 5-20 ug/mL
Immunocytochemistry: 5-20 ug/mL
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20 ug/mL
Immunocytochemistry: 5-20 ug/mL
Optimal working dilutions must be determined by end user.
Organism Species
Mus musculus (Mouse)
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant ESAM (Ala29~Ser248) expressed in E.coli
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2074560
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 24 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 24 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Figure. Western Blot; Sample: Recombinant ESAM, Mouse.)
Western Blot (WB)
(Figure. Western Blot; Sample: Recombinant ESAM, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Small intestine Tissue.Primary Ab: 20 ug/ml Rabbit Anti-Mouse ESAM AntibodySecond Ab: 2 ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: MBS2086047))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Small intestine Tissue.Primary Ab: 20 ug/ml Rabbit Anti-Mouse ESAM AntibodySecond Ab: 2 ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: MBS2086047))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
endothelial cell-selective adhesion molecule
NCBI Official Synonym Full Names
endothelial cell-specific adhesion molecule
NCBI Official Symbol
Esam
NCBI Official Synonym Symbols
Esam1; W117m; 2310008D05Rik
NCBI Protein Information
endothelial cell-selective adhesion molecule
UniProt Protein Name
Endothelial cell-selective adhesion molecule
Protein Family
UniProt Gene Name
Esam
UniProt Synonym Gene Names
Esam1
Uniprot Description
ESAM: Can mediate aggregation most likely through a homophilic molecular interaction.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 9|9 A4
Cellular Component: adherens junction; bicellular tight junction; cell junction; integral component of membrane; membrane; plasma membrane
Biological Process: calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules; cell adhesion; homophilic cell adhesion
Research Articles on ESAM
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Product Notes
The ESAM esam (Catalog #AAA2004628) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Endothelial Cell Adhesion Molecule (ESAM) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Endothelial Cell Adhesion Molecule (ESAM) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western blotting: 0.5-2 ug/mL Immunohistochemistry: 5-20 ug/mL Immunocytochemistry: 5-20 ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the ESAM esam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Endothelial Cell Adhesion Molecule (ESAM), Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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