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Western Blot (WB) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GPD1L antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : MCF7 cell lysate Lane 2. : 293T cell lysate Lane 3. : Jurkat cell lysate Lane 4 : SW480 cell lysate Lane 5 : PC3 cell lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GPD1L antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : 293T cell lysate Lane 2. : GPD1L Transfected 293T cell lysate )

Mouse anti-Human GPD1L Monoclonal Antibody | anti-GPD1L antibody

GPD1L antibody

Gene Names
GPD1L; GPD1-L
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
Purity
By protein-A affinity chromatography
Synonyms
GPD1L; Monoclonal Antibody; GPD1L antibody; Glycerol-3-phosphate dehydrogenase 1-like protein; GPD1-L; anti-GPD1L antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT14E2
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
351
Applicable Applications for anti-GPD1L antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Antigen Species
Human
Immunogen
Recombinant human GPD1L (1-351aa) purified from E. coli
Clone Note
Anti-human GPD1L mAb, clone AT14E2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human GPD1L protein
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.

Western Blot (WB)

(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GPD1L antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : MCF7 cell lysate Lane 2. : 293T cell lysate Lane 3. : Jurkat cell lysate Lane 4 : SW480 cell lysate Lane 5 : PC3 cell lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GPD1L antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : 293T cell lysate Lane 2. : GPD1L Transfected 293T cell lysate )

Western Blot (WB) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GPD1L antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : MCF7 cell lysate Lane 2. : 293T cell lysate Lane 3. : Jurkat cell lysate Lane 4 : SW480 cell lysate Lane 5 : PC3 cell lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GPD1L antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : 293T cell lysate Lane 2. : GPD1L Transfected 293T cell lysate )

Immunofluorescence (IF)

(ICC/IF analysis of GPD1L in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GPD1L antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)

Immunofluorescence (IF) (ICC/IF analysis of GPD1L in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GPD1L antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Related Product Information for anti-GPD1L antibody
GPD1L is converts sn-glycerol 3-phosphate to glycerone phosphate. This protein is found in the cytoplasm, associated with the plasma membrane, where it binds the sodium channel, voltage-gated, type V, alpha subunit (SCN5A). GPD1L is thought to affect trafficking of the cardiac sodium current to the cell surface. Mutations in GPD1L are the cause of sudden infant death syndrome (SIDS) and Brugada syndrome type 2, an autosomal dominant tachyarrhythmia.
Product Categories/Family for anti-GPD1L antibody
References
Valdivia CR., et al. (2009) Am. J. Physiol. 297: H1446-H1452.; Van Norstrand DW., et al. (2007) Circulation. 116: 2253-2259.;

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38,419 Da
NCBI Official Full Name
glycerol-3-phosphate dehydrogenase 1-like protein
NCBI Official Synonym Full Names
glycerol-3-phosphate dehydrogenase 1 like
NCBI Official Symbol
GPD1L
NCBI Official Synonym Symbols
GPD1-L
NCBI Protein Information
glycerol-3-phosphate dehydrogenase 1-like protein
UniProt Protein Name
Glycerol-3-phosphate dehydrogenase 1-like protein
UniProt Gene Name
GPD1L
UniProt Synonym Gene Names
KIAA0089; GPD1-L

NCBI Description

The protein encoded by this gene catalyzes the conversion of sn-glycerol 3-phosphate to glycerone phosphate. The encoded protein is found in the cytoplasm, associated with the plasma membrane, where it binds the sodium channel, voltage-gated, type V, alpha subunit (SCN5A). Defects in this gene are a cause of Brugada syndrome type 2 (BRS2) as well as sudden infant death syndrome (SIDS). [provided by RefSeq, Jul 2010]

Uniprot Description

Plays a role in regulating cardiac sodium current; decreased enzymatic activity with resulting increased levels of glycerol 3-phosphate activating the DPD1L-dependent SCN5A phosphorylation pathway, may ultimately lead to decreased sodium current; cardiac sodium current may also be reduced due to alterations of NAD(H) balance induced by DPD1L.

Research Articles on GPD1L

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Product Notes

The GPD1L gpd1l (Catalog #AAA200414) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GPD1L antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GPD1L can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the GPD1L gpd1l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPD1L, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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