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Western Blot (WB)
(Western Blot: Sample: Human Saliva; Primary Ab: 5ug/ml Rabbit Anti-Rat LPO Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Rat Lactoperoxidase (LPO) Polyclonal Antibody | anti-LPO antibody
Polyclonal Antibody to Lactoperoxidase (LPO)
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Lactoperoxidase (LPO); Polyclonal Antibody; Polyclonal Antibody to Lactoperoxidase (LPO); anti-LPO antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against LPO. It has been selected for its ability to recognize LPO in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF-PCEVINATAG VPCFLAGDSR ASEQILLATS HTLFIREHNR LARELSTLNP HWDGETLYQE TRKIMGAFIQ IITFRDYLPI LLGDEMQKWI PPYQGYNESV DPRISNVFTF AFRFGHLEIP STVSRLDENY QPWGSEPELP LHTVFFNTWR LVKDGGIDPL VRGLLAKKAK LMHQDRMMTG ELRNKLFQPT HTIHGFDLAS INIQRCRDHG MPGYNSWRAF CGLSQPKTLE ELSAVMENE
MGHHHHHHSGSEF-PCEVINATAG VPCFLAGDSR ASEQILLATS HTLFIREHNR LARELSTLNP HWDGETLYQE TRKIMGAFIQ IITFRDYLPI LLGDEMQKWI PPYQGYNESV DPRISNVFTF AFRFGHLEIP STVSRLDENY QPWGSEPELP LHTVFFNTWR LVKDGGIDPL VRGLLAKKAK LMHQDRMMTG ELRNKLFQPT HTIHGFDLAS INIQRCRDHG MPGYNSWRAF CGLSQPKTLE ELSAVMENE
Sequence Length
324
Applicable Applications for anti-LPO antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant LPO (Pro351~Glu589) expressed in E.coli.
Cross Reactivity
Human, Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2040677
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Human Saliva; Primary Ab: 5ug/ml Rabbit Anti-Rat LPO Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human Saliva; Primary Ab: 5ug/ml Rabbit Anti-Rat LPO Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Stomach Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Stomach Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
78,248 Da
NCBI Official Full Name
lactoperoxidase
NCBI Official Synonym Full Names
lactoperoxidase
NCBI Official Symbol
Lpo
NCBI Protein Information
lactoperoxidase
UniProt Protein Name
Lactoperoxidase
Protein Family
UniProt Gene Name
Lpo
NCBI Description
This gene encodes an oxidoreductase secreted from salivary, mammary, and other mucosal glands that functions as a natural antibacterial agent. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
Uniprot Description
LPO: May contribute to airway host defense against infection. Belongs to the peroxidase family. XPO subfamily.
Protein type: Secreted, signal peptide; EC 1.11.1.7; Secreted; Oxidoreductase
Chromosomal Location of Human Ortholog: 17q23.1
Cellular Component: extracellular space; basolateral plasma membrane; cytoplasm
Molecular Function: metal ion binding; heme binding
Biological Process: hydrogen peroxide catabolic process; defense response to bacterium; thiocyanate metabolic process; detection of chemical stimulus involved in sensory perception of bitter taste; response to oxidative stress
Research Articles on LPO
Similar Products
Product Notes
The LPO lpo (Catalog #AAA2003745) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Lactoperoxidase (LPO) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Lactoperoxidase (LPO) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the LPO lpo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-PCEVIN ATAG VPCFLAGDSR ASEQILLATS HTLFIREHNR LARELSTLNP HWDGETLYQE TRKIMGAFIQ IITFRDYLPI LLGDEMQKWI PPYQGYNESV DPRISNVFTF AFRFGHLEIP STVSRLDENY QPWGSEPELP LHTVFFNTWR LVKDGGIDPL VRGLLAKKAK LMHQDRMMTG ELRNKLFQPT HTIHGFDLAS INIQRCRDHG MPGYNSWRAF CGLSQPKTLE ELSAVMENE. It is sometimes possible for the material contained within the vial of "Lactoperoxidase (LPO), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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