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Western Blot (WB) (Western Blot: Sample: Human Lung lysate; Primary Ab: 1ug/ml Rabbit Anti-Human QSOX1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Rabbit anti-Human Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1) Polyclonal Antibody | anti-QSOX1 antibody

Polyclonal Antibody to Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1)

Gene Names
QSOX1; Q6; QSCN6
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1); Polyclonal Antibody; Polyclonal Antibody to Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1); anti-QSOX1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against QSOX1. It has been selected for its ability to recognize QSOX1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF-SPSD PLTLLQADTV RGAVLGSRSA WAVEFFASWC GHCIAFAPTW KALAEDVKAW RPALYLAALD CAEETNSAVC RDFNIPGFPT VRFFKAFTKN GSGAVFPVAG ADVQTLRERL IDALESHHDT WPPACPPLEP AKLEEIDGFF A
Sequence Length
747
Applicable Applications for anti-QSOX1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant QSOX1 (Ser37~Ala181) expressed in E.coli.
Cross Reactivity
Human
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2068037
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Human Lung lysate; Primary Ab: 1ug/ml Rabbit Anti-Human QSOX1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Human Lung lysate; Primary Ab: 1ug/ml Rabbit Anti-Human QSOX1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded liver tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin- embedded liver tissue))

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Liver Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Liver Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
66,861 Da
NCBI Official Full Name
sulfhydryl oxidase 1 isoform b
NCBI Official Synonym Full Names
quiescin sulfhydryl oxidase 1
NCBI Official Symbol
QSOX1
NCBI Official Synonym Symbols
Q6; QSCN6
NCBI Protein Information
sulfhydryl oxidase 1
UniProt Protein Name
Sulfhydryl oxidase 1
Protein Family
UniProt Gene Name
QSOX1
UniProt Synonym Gene Names
QSCN6; hQSOX

NCBI Description

This gene encodes a protein that contains domains of thioredoxin and ERV1, members of two long-standing gene families. The gene expression is induced as fibroblasts begin to exit the proliferative cycle and enter quiescence, suggesting that this gene plays an important role in growth regulation. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

quiescin Q6: Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide. May contribute to disulfide bond formation in a variety of secreted proteins. In fibroblasts, it may have tumor- suppressing capabilities being involved in growth regulation. Belongs to the quiescin-sulfhydryl oxidase (QSOX) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.8.3.2; Membrane protein, integral; Oxidoreductase

Chromosomal Location of Human Ortholog: 1q25.2

Cellular Component: endoplasmic reticulum lumen; extracellular exosome; extracellular region; extracellular space; Golgi apparatus; integral to Golgi membrane; intercellular bridge; intracellular membrane-bound organelle; tertiary granule lumen

Molecular Function: flavin-linked sulfhydryl oxidase activity; protein disulfide isomerase activity

Biological Process: cell redox homeostasis; cellular protein metabolic process; negative regulation of macroautophagy; neutrophil degranulation; platelet degranulation; post-translational protein modification

Research Articles on QSOX1

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Product Notes

The QSOX1 qsox1 (Catalog #AAA2003269) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the QSOX1 qsox1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-SPSD PLTLLQADTV RGAVLGSRSA WAVEFFASWC GHCIAFAPTW KALAEDVKAW RPALYLAALD CAEETNSAVC RDFNIPGFPT VRFFKAFTKN GSGAVFPVAG ADVQTLRERL IDALESHHDT WPPACPPLEP AKLEEIDGFF A. It is sometimes possible for the material contained within the vial of "Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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